新疆小麦品种(系)高分子量麦谷蛋白亚基遗传多样性分析

[目的]更好的了解和有效利用新疆选育的小麦种质材料.[方法]采用SDS-PAGE技术对88份小麦育成品种与自育品系的高分子量麦谷白亚基(HMW-GS)组成进行了分析.[结果]参试材料中共有15种HMW-GS类型,Glu-A1位点上有、1和2~*,以亚基为主要类型(占57.96; );Giu-B1位点上有7+9、7+8、6+8、14+15、17+18、7、8和20八种类型,以7+9和7+8为主要亚基类型(分别占45.46;和39.77;);Glu-D1位点上有5+10、2+12、3+12和2+11四种类型,其中2+12亚基的频率高达59.09;;88份小麦品种(系)的亚基组合类型共有25种,其中主要亚基组合为”,7+8,2+12",占17.05; .通过对比新疆育成小麦品种和自育品系的优质亚基出现频率发现,品系中优质亚基1、14+15和5+10出现频率较育成品种有所增长,2~*、7+8、17+18优质亚基的出现频率下降.在参试材料中,32个含有5+10优质亚基,有21个含有1亚基,6个含2~*亚基,1个含14+15亚基,1个含17+18亚基,并有两个材料检测出优质亚基组合类型(”2~*、17+18、5+10",”1、7+8、5+10").[结论]这些优异种质资源可供优质小麦育种利用.

Analysis of Genetic Diversity of HMW-GS in Wheat Varieties and Strains of Xinjiang

[Objective]The high-molecular-weight gluten subunits(HMW-GS)of [Method]88 wheat cultivars were analyzed by means of SDS-PAGE in order to make good use of crop germplasms resource of Xinjiang,[Result]a total of 15 HMW-GS pattern were identified.At Glu-A1 loci,3 alleles were detected,which encoded subunit 1,2~* and Null respectively and the major subunit was Null with the 57.96 .At Glu-B1 loci,8 alleles were detected,which encoded subunits 7+8,7+9 ,6+8,14+15,17+18,7,8 and20 ,7+8 and 7+9 were the major subunit patterns(45.46%,39.77%).4 alleles encoded subunits 5+10,2+12 ,3+12 and 2+11respectively were detected at Glu-D1 loci and 2+12 was the major subunit pattern(59.09%).There were 25 subunits combination patterns in wheat cultivars from Xinjiang wheat and the major pattern was"Null,7+8,2+12"with the frequency of 17.05.The frequency of high-quality HMW-GS(1,14+15,5+10) of strains was risen compared with early phase varieties,and the appearance rate of high-quality from other varielies (2~*,7+8,17+18) fell off to some extent. Among 88 wheat germplasm,32 carried 5+10 high-quality subunits,21 of that had 1 subunits,6 of that had 2~* subunits,1 of that had 14+15 subunits,1 of that had 17+18 subunits,and 2 of that had high quality subunits combinations(2~*,17+18,5+10,1,7+8,5+10),[Conclusion]these crop germplasms were utilized in wheat quality breeding.