| 卵形鲳鲹结节病病原的分离与鉴定 |
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| 引用本文： | 陈建林, 苏泽杰, 鲁义善, 徐亮, 张红莲, 夏立群. 鰑鱼诺卡氏菌HYD基因的克隆及亚细胞定位研究[J]. 南方水产科学, 2018, 14(1): 35-42. DOI: 10.3969/j.issn.2095-0780.2018.01.005 |
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| 作者姓名： | 陈建林 苏泽杰 鲁义善 徐亮 张红莲 夏立群 |
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| 作者单位： | 1.广东海洋大学深圳研究院，广东 深圳 518108;2.广东海洋大学水产学院，广东 湛江 524088;3.广东省水产经济动物病原生物学及流行病学重点实验室，广东 湛江 524088 |
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| 基金项目： | 深圳市科技计划项目 (JCYJ20170306161613251)；广东省自然科学基金 (2017A030313179)；广东省科技发展专项资金项目(2016A050502061)；深圳大鹏新区产业发展专项资金项目(KY20160207)；广东海洋大学自然科学研究项目(C13454，E10085) |
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| 摘 要： | 鲪鱼诺卡氏菌(Nocardia seriolae)是鱼类诺卡氏菌病的主要病原。鲪鱼诺卡氏菌水解酶(hydrolase，HYD)可能是鲪鱼诺卡氏菌的毒力因子。研究结果表明鲪鱼诺卡氏菌HYD的肽段不是分泌蛋白。亚细胞定位研究发现HYD-GFP融合蛋白均匀地分布在胖头鲤(FHM)细胞的细胞质中。亚细胞定位和过表达研究都显示HYD蛋白在FHM细胞中表达后，细胞核出现固缩浓染、凋亡小体等细胞凋亡特征，但Caspase-3活性检测表明HYD并未显著增加细胞凋亡水平。研究为进一步了解鲪鱼诺卡氏菌的致病机理奠定了基础。
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| 关 键 词： | 鲪鱼诺卡氏菌 水解酶 亚细胞定位 Caspase-3活性检测 |
| 收稿时间： | 2017-06-20 |
| 修稿时间： | 2017-09-12 |
Isolation and genetic characterization of Nocardia seriolae from snubnose pompano Trachinotus blochii in Vietnam |
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| CHEN Jianlin, SU Zejie, LU Yishan, XU Liang, ZHANG Honglian, XIA Liqun. Cloning and subcellular localization of HYD gene from Nocardia seriolae[J]. South China Fisheries Science, 2018, 14(1): 35-42. DOI: 10.3969/j.issn.2095-0780.2018.01.005 |
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| Authors: | CHEN Jianlin SU Zejie LU Yishan XU Liang ZHANG Honglian XIA Liqun |
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| Affiliation: | 1.Shenzhen Research Institute of Guangdong Ocean University, Shenzhen 518108, China;2.Fisheries College of Guangdong Ocean University, Zhanjiang 524088, China;3.Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Zhanjiang 524088, China |
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| Abstract: | Nocardia seriolae is the main pathogen of fish nocardiosis. N.seriolae hydrolase (HYD) may be the virulence factor of N.seriolae. In this study, the results show that the N.seriolae HYD is not a secreted protein. Subcellular localization studies reveal that HYD-GFP fusion proteins uniformly distributed in whole FHM cell. Typical apoptotic features, such as nuclear pyrosis and apoptotic bodies, were found when HYD protein expressed in FHM cells according to subcellular localization and overexpression studies, while the quantitative assay of Caspase-3 activity shows that HYD did not increase the level of apoptosis significantly. The study lays foundation for further study on the pathogenic mechanism of N.seriolae. |
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| Keywords: | Nocardia seriolae HYD subcellular localization Caspase-3 assay |
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