| 栉孔扇贝骨形态发生蛋白2基因的克隆及表达分析 |
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| 引用本文： | 范嗣刚, 周代志, 刘宝锁, 邓正华, 郭奕惠, 喻达辉. 合浦珠母贝BMP7b基因的克隆与表达分析[J]. 南方水产科学, 2018, 14(1): 121-126. DOI: 10.3969/j.issn.2095-0780.2018.01.016 |
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| 作者姓名： | 范嗣刚 周代志 刘宝锁 邓正华 郭奕惠 喻达辉 |
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| 作者单位： | 1.中国水产科学研究院南海水产研究所，农业部南海渔业资源开发利用重点实验室，农业部水产品加工重点实验室，广东 广州 510300;2.钦州学院，广西 钦州 535011 |
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| 基金项目： | 国家自然科学基金项目 (31372525)；广东省自然科学基金项目 (2014A030310237，2016A030313142)；广东省海洋渔业科技与产业发展专项(B201601-Z01，B201601-Z02)；中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2015TS28) |
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| 摘 要： | 为了解骨形态发生蛋白(BMP)在贝类生物矿化方面的作用，该研究发现并克隆了合浦珠母贝(Pinctada fucata) BMP7的同源基因BMP7b，并对其进行了组织表达分析。结果显示，该基因cDNA长2 464 bp，其中ORF长1 194 bp，编码397个氨基酸。BMP7b蛋白无跨膜结构，含有1个信号肽(1~26 aa)，1个TGF-β前肽结构域(25~240 aa)和1个TGF-β结构域(299~396 aa)。BMP7b在各组织和各胚胎发育时期中均有表达，在鳃和外套膜的表达量高，在肠和肝胰腺的表达量低；在担轮幼虫期的表达量远远高于其他时期。贝壳缺刻实验显示24 h后，BMP7b表达量显著上升。以上结果表明BMP7b可能在合浦珠母贝贝壳形成和修护过程中起重要作用。
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| 关 键 词： | 合浦珠母贝 BMP7b 时空表达模式 贝壳形成与修复 |
| 收稿时间： | 2017-08-11 |
| 修稿时间： | 2017-09-13 |
Signal transduction by bone morphogenetic proteins |
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| FAN Sigang, ZHOU Daizhi, LIU Baosuo, DENG Zhenghua, GUO Yihui, YU Dahui. Molecular cloning and expression analysis of BMP7b from Pinctada fucata[J]. South China Fisheries Science, 2018, 14(1): 121-126. DOI: 10.3969/j.issn.2095-0780.2018.01.016 |
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| Authors: | FAN Sigang ZHOU Daizhi LIU Baosuo DENG Zhenghua GUO Yihui YU Dahui |
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| Affiliation: | 1.Key Laborotary of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; Key Laborotary of Aquatic Product Processing, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;2.Qinzhou University, Qinzhou 535011, China |
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| Abstract: | In order to understand the role of BMP in biomineralization process in mollusca, a BMP7 homolog in Pinctada fucata named BMP7b was cloned in this study. The full length of BMP7b was 2 464 bp, including an open reading frame (ORF) of 1 194 bp which encoded 397 amino acids. The protein sequence included a signal peptide (1–26 aa), a pro-domain (25–240 aa) and a TGF-β domain (299–396 aa). No transmembrane domain was detected. BMP7b was expressed in all kinds of tissues and was higher expressed in gill and mantle, but lower in intestine and hepatopancreas. The BMP7b expression level at larva stage was much higher than that in other periods. In shell notch experiment, BMP7b showed increasing expression level after 24 h. These results indicate that BMP7b may play an important role in process of shell formation and repairment. |
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| Keywords: | Pinctada fucata BMP7b temporal spatial expression shell formation and repair |
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