| 甘蔗叶片蔗糖磷酸合成酶基因cDNA克隆及序列分析 |
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| 引用本文： | 何文锦,黄金存,周平,叶冰莹,陈由强,陈如凯.甘蔗叶片蔗糖磷酸合成酶基因cDNA克隆及序列分析[J].亚热带农业研究,2007,3(4):294-296. |
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| 作者姓名： | 何文锦 黄金存 周平 叶冰莹 陈由强 陈如凯 |
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| 作者单位： | 1. 福建师范大学生命科学学院,福建福州,350108;福建师范大学农业部甘蔗生理生态与遗传改良重点开放室验室,福建福州,350108
2. 福建师范大学生命科学学院,福建福州,350108;福建师范大学农业部甘蔗生理生态与遗传改良重点开放室验室,福建福州,350108;发育与神经生物学福建省高等学校重点实验室,福建福州,350108 |
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| 基金项目： | 引进国际先进农业科技计划(948计划)
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卫生部资助项目
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福建省教育厅科研项目 |
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| 摘 要： | 蔗糖磷酸合成酶(sucrose phosphate synthase,SPS)是高等植物体内控制蔗糖合成的关键酶之一。根据已报道的甘蔗蔗糖磷酸合成酶基因SoSPS2(GenBank登录号:AB001338.1)序列,采用RT-PCR方法从甘蔗(Saccharum officinarum FN95-1702)叶片中克隆获得SPS基因的cDNA片段。测序结果表明,该cDNA长3013 bp,其中第59-2953位是该基因的开放阅读框(ORF),编码964个氨基酸。与GenBank上已公布的序列比对分析表明,二者序列相似性达99.07%,氨基酸序列相似性达98.86%。
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| 关 键 词： | 蔗糖磷酸合成酶 甘蔗 cDNA |
| 文章编号： | 1673-0925(2007)04-0294-03 |
| 修稿时间： | 2007年9月30日 |
Cloning and sequence analysis of sucrose phosphate synthase cDNA from sugarcane leaf |
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| HE Wen-jin,HUANG Jin-cun,ZHOU Ping,YE Bing-ying,CHEN You-qiang,CHEN Ru-kai.Cloning and sequence analysis of sucrose phosphate synthase cDNA from sugarcane leaf[J].Subtropical Agriculture Research,2007,3(4):294-296. |
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| Authors: | HE Wen-jin HUANG Jin-cun ZHOU Ping YE Bing-ying CHEN You-qiang CHEN Ru-kai |
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| Abstract: | Sucrose phosphate synthase(SPS) is one of the key enzymes controlling biosynthesis of sucrose in higher plants.According to the published sequence of sucrose phosphate synthase gene(SoSPS2,AB001338.1) shown to express in sugarcane,a cDNA fragment of SPS gene was cloned from sugarcane(Saccharum officinarum FN95-1702) leaf by RT-PCR.The sequencing result is that the length of the cDNA fragment is 3013 bp,whose ORF ranges from position 59 to 2953,encoding 964 amino acids.Alignment of the cloned SPS sequence with the published sequence from GenBank revealed 99.07% similarity in nucleotides and 98.86% similarity in deduced amino acid sequence. |
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| Keywords: | sucrose phosphate synthase(SPS) sugarcane cDNA |
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