Intraradical hyphae phosphatase of the arbuscular mycorrhizal fungus, Gigaspora margarita |
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Authors: | T Kojima M Hayatsu M Saito |
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Institution: | (1) National Grassland Research Institute, Nishinasuno, Tochigi, 329-2793, Japan Tel: +81-287-37-7227; Fax: +81-287-36-6629; e-mail: kojima@ngri.affrc.go.jp, JP |
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Abstract: | An alkaline phosphatase in the intraradical hyphae of arbuscular mycorrhizal fungi was found to be closely related to an
improvement of plant growth. To detect the phosphatase activity in a crude extract of mycorrhizal roots, phosphatase isozymes
in mycorrhizal and non-mycorrhizal onion roots were compared with those in Gigaspora margarita by electrophoresis. A mycorrhiza-specific band was found when the phosphatase was stained under alkaline conditions. To clarify
the origin of this phosphatase, the phosphatase extracted from intraradical hyphae was also compared with the phosphatase
from mycorrhizal roots by electrophoresis. The intraradical hyphae was isolated from mycorrhizal roots by enzyme digestion
followed by Percoll gradient centrifugation. The soluble protein was extracted from the hyphae by ultra-sonication after treatment
with chitinase. A phosphatase in the hyphal soluble protein showed a similar, but slightly higher, relative mobility on the
gel, compared with the mycorrhiza-specific phosphatase from roots. By adding the hyphal extract to the root extract, the relative
mobility of the mycorrhiza-specific phosphatase was slightly changed and became identical to that of the phosphatase in the
hyphae. This indicated that the specific band of phosphatase found in the crude extract from mycorrhizal roots was of intraradical
hyphal origin.
Received: 16 April 1997 |
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Keywords: | Mycorrhiza-specific phosphatase Intraradical hyphae Gigaspora margarita Onion |
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