Effect of density gradient composition on in vitro maturation of stallion sperm |
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| Authors: | JL Turner MS MJ Arns PhD |
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| Institution: | 1. ANOVA – Clinic for Endocrinology, Karolinska University Hospital and Department of Medicine, Huddinge, Karolinska Institutet, Stockholm, Sweden;2. Centre for Human Reproductive Science, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom;3. Other Editorial Board Members and Contributors of the WHO Laboratory Manual for the Examination and Processing of Human Semen, 6th edition;1. Department of Obstetrics and Gynecology, Institute of Clinical Science, Sahlgrenska Academy, Gothenburg University, Reproductive Medicine, Gothenburg, Sweden;2. East Hospital, Sahlgrenska University Hospital, Gothenburg, Sweden;3. Department of Reproduction Epidemiology, Tornblad Institute, Institution of Clinical Science, Lund University, Lund, Sweden;4. Fertility Center, Carlanderska Hospital, Gothenburg, Sweden;5. In Vitro Fertilization Clinic, Falun, Sweden;1. Department of Gynecology and Obstetrics, Division of Maternal-Fetal Medicine, Johns Hopkins University School of Medicine, Baltimore, MD;2. Department of Population, Family, and Reproductive Health, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University School of Medicine, Baltimore, MD;3. Neuroscience Intensive Care Nursery Program, Johns Hopkins University School of Medicine, Baltimore, MD;4. Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD;5. Integrated Research Center for Fetal Medicine, Johns Hopkins University School of Medicine, Baltimore, MD;6. Department of Pediatrics, Division of Neonatology, Johns Hopkins University School of Medicine, Baltimore, MD;1. Fundación Universitaria Sánitas, Bogotá D.C., Colombia;2. E.S.E. Centro Dermatológico Federico Lleras Acosta, Bogotá D.C., Colombia;3. E.S.E. Instituto Nacional de Cancerología, Bogotá D.C., Colombia;4. Hospital Universitario Fundación Santa Fe, Bogotá D.C., Colombia |
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| Abstract: | The present study was conducted to determine the influence of density gradient composition on in vitro capacitation of stallion spermatozoa. In Experiment I spermatozoa were isolated on either 90% Percoll (P), 90% arabinogalactan (AG), or 12% BSA gradients and then challenged with 1 μM A23187 (15, 150, and 270 min) and heat-solubilized equine zonae pellucidae (270 min, sEZP, 2 ZP/μl). The P gradient enhanced the percentage of progressively motile spermatozoa (PMS) more (P=.0001) than AG or BSA gradients immediately post-processing, but was not sustained throughout the culture period. The viability for P-separated spermatozoa was higher (P=.01) than that of BSA or AG-separated spermatozoa. Gradient composition had no effect (P=.68) on the percentage of live, acrosome reacted spermatozoa (PAR), before and following ionophore and sEZP challenge. In Experiment II, the number of spermatozoa penetrating the ZP of saltstored oocytes was not influenced (P=.35) by gradient composition; however, the number of spermatozoa bound per oocyte was higher (P=.02) for P-separated spermatozoa than for AG or BSA-separated spermatozoa. These data suggest that isolation on a P density gradient may enhance in vitro capacitation of stallion spermatozoa. |
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