| 四唑染色法测定毛竹种子生活力的研究 |
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| 引用本文： | 蔡春菊,彭镇华,高志民,高健,李雪平.四唑染色法测定毛竹种子生活力的研究[J].世界竹藤通讯,2008,6(3):10-13. |
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| 作者姓名： | 蔡春菊 彭镇华 高志民 高健 李雪平 |
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| 作者单位： | 国际竹藤网络中心 国家林业局竹藤科学与技术重点实验室 北京 100102 |
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| 基金项目： | 国家科技支撑项目
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国际竹藤网络中心基本科研业务费专项 |
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| 摘 要： | 采用染色全过程观察和正交试验方法研究了TTC浓度、温度和染色时间对毛竹种子生活力的影响,探讨毛竹种子生活力测定的最佳条件。结果表明:四唑染色法能够很好地检测毛竹种子生活力水平,估测种子的潜在发芽能力。TTC浓度、温度和染色时间在生活力测定中存在一定的线性关系。试验表明,25℃下浸种48 h待种子完全吸涨后,于30~35℃黑暗条件下、TTC浓度0.5%,染色3~4 h为毛竹种子生活力测定的最佳条件。
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| 关 键 词： | 毛竹种子 生活力 四唑测定技术 |
Study on Moso Seed Viability by TTC Testing Method |
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| Cai Chunju,Peng Zhenhua,Gao Zhimin,Gao Jian,Li Xueping.Study on Moso Seed Viability by TTC Testing Method[J].World Bamboo and Rattan,2008,6(3):10-13. |
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| Authors: | Cai Chunju Peng Zhenhua Gao Zhimin Gao Jian Li Xueping |
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| Institution: | International Center for Bamboo and Rattan, Key Laboratory of Science and Technology of Bamboo and Rattan, State Forestry Administration, Beijing 100102 |
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| Abstract: | To find the optimum conditions for testing seed viabilities of Phyllostachys edulis, TTC concentration, dyeing temperature and dyeing time were tested by dyeing process observation and orthogonal design（four levels in each of three factors）. The results showed that TTC methods could test seed viability level.effectively and appraise potential germination ability of Phyllostachys edulis. There existed linear relationship among solution concentration, dyeing temperature and dyeing time. This paper proposed that after full imbibition treatment by dipping in water for 48 hours at 25℃, 0.5% of TTC concentration, 30-35℃ of temperature and 3-4 h of dyeing time in darkness could be the best condition for Phyllostachys edulis seed viability testing. |
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| Keywords: | seed Phyllostachys edulis viability TTC testing method |
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