肿柄菊氯仿萃取相化学成分分析及其植物毒性

采用GC-MS联用技术对肿柄菊氯仿萃取相化学成分进行分析,并以白菜Brassica pekinensis (Lour.) Rupr]、萝卜 (Raphanus sativus Linnaeus)和莴苣(Lactuca sativa Linnaeus)为供试植物,采用培养皿滤纸法对不同质量浓度(0.1、0.05、0.025 g/mL)肿柄菊氯仿萃取相进行了生物测定.结果表明,肿柄菊氯仿萃取相化学成分中共鉴定了48个化合物,占其总量的81.28％.肿柄菊氯仿萃取相化学成分主要为醇类、酯类、菇类等,其中含量较高的依次为豆甾醇(10.335％)、穿贝海绵甾醇(8.640％)、十六酸乙酯(7.164％)、7-tert-butyl-1-tetralone( 6.304％)、2-单棕榈酸甘油(4.226％)、新植二烯(4.089％)等.通过种子萌发和幼苗生长抑制试验发现,肿柄菊氯仿萃取相对白菜、萝卜和莴苣种子的最终发芽率及幼苗生长总体上均产生抑制作用,并且随质量浓度的增大,抑制作用增强,其中肿柄菊氯仿萃取相对白菜种子萌发和莴苣幼苗生长影响较为明显.

Chemical Composition of Chloroform-extraction from Tithonia diversifolia and Its Phytotoxicity

The chemical composition of the chloroform-extraction from Tithonia diversifolia was analyzed with GC-MS, and the effect of the chemical composition of chloroform-extraction with different concentrations(0.1, 0.05 and 0.025 g/mL)was bioassayed on B. pekinensis, R. sativus, L. sativa by culture dish method. 48 compounds were identified, amouting to 81.28%. The major constituents were alcohols, esters, terpenes. Stigmasterol(10.335%), Clionasterol(8.640%), Ethyl palmitate(7.164%), 7-tert-butyl-1- tetralone(6.304%), 2-momoPalmitin(4.226%), Neophytadiene(4.089%)were the main compounds from the chloroform-extraction. The results showed that the chemical composition of the chloroform-extraction from T. diversifolia could inhibit final seed germination rate, seedling growth. The suppression effect was strengtheded with the increasing concentration, and the chloroform-extraction fraction had an obvious inhibition effect on. B. pekinensis seed germination and L. sativa seedling growth.