大豆种子吸胀时内含物外渗及其与种子活力的相关性研究

大豆种子吸胀期间有明显的溶质外渗现象,本研究表明:种子在低渗透势溶液中吸胀,有利于细胞膜系统的修复和完整性建立,其外渗液的相对电导和[K~+]较低,种子活力指数提高;种子在-1.1860 MPa溶液中吸胀48小时效果最佳,此时相对电导为4.3％,[K~+]为42ppm,活力指数为1.168;外渗液的相对电导和[K~+]与活力指数之间呈Y=1/(a+bx)的函数关系,相关系数分别为0.9847和0.9548,均达极显著水平,两者可作为种子活力的测定方法。

STUDIES ON SOLUTE EXUDATE DURING SOYBEAN SEED IMBIBITION AND RELATION WITH SEED VIGOR

There was evident solute exudation during soybean seed imbibition. The results showed the that seed imbibing in low potential solution were conducive to the repair and formation of integrity of plasma membrane, resulting in the decrease of relative eletro-conductivity and [K+] of exndate and the increase of seed vigor in index. Seeds imbibing in - 1.1860MPa solution for 48 hours have best effect in the experiment, with relative alactro-conductivity and [K+] of seed exudate after treatment being 4.3% and 42ppm, vigor index being 1.168. The study also indi-uatsd that there was the relation of Y=1/a+bx between relative electro-conductivity and [K+]of exudate and vigor index of seeds, the correlation coefficients between them were 0. 984 and 0. 9548 respectively. They were good methods for determining seed vigor.