|
|||||
|
|
| 犬瘟热病毒单克隆抗体的制备及特性鉴定 | |
| 引用本文： | 苏建青,岳妙姝,王化磊,薛琳,夏咸柱.犬瘟热病毒单克隆抗体的制备及特性鉴定[J].吉林农业大学学报,2009,31(1). |
| 作者姓名： | 苏建青 岳妙姝 王化磊 薛琳 夏咸柱 |
| 作者单位： | 1. 吉林大学畜牧兽医学院,长春,130062;解放军军事医学科学院军事兽医研究所,长春,130062;聊城大学农学院,聊城,252059 2. 长春理工大学生命科学技术学院,长春,130062 3. 解放军军事医学科学院军事兽医研究所,长春,130062 |
| 基金项目： | 解放军总后勤部科技攻关项目 |
| 摘 要： | 用纯化的犬瘟热病毒免疫Balb/C小鼠,采用问接ELJSA方法筛选分泌阳性抗体的杂交瘤细胞株,挑选阳性孔进行亚克隆,用Vero细胞交叉反应试验及间接免疫荧光试验检测单抗的特异性.结果获得6株能稳定分泌抗犬瘟热病毒的单克隆抗体细胞株,分别命名为AD7、AE2、CE3、EH5、GG6和GF1.交叉反应试验及间接免疫荧光染色检测表明,6株单抗的特异性良好;经过体外连续传代及反复冻存、复苏,杂交瘤细胞均能稳定分泌特异性单抗.培养上清液及小鼠腹水ELISA效价分别为26～211和104～107.相加试验表明:单抗AE2、CE3、GG6和GF1具有共同的表位,而单抗AD7和EH5识别的位点与其他4株不同. |
| 关 键 词： | 犬瘟热病毒 单克隆抗体 间接酶联免疫吸附试验 |
Generation and Characterization of Monoclonal Antibodies against Canine Distemper Virus |
|
| SU Jian-qing,YUE Miao-shu,WANG Hua-lei,XUE Lin,XIA Xian-zhu.Generation and Characterization of Monoclonal Antibodies against Canine Distemper Virus[J].Journal of Jilin Agricultural University,2009,31(1). | |
| Authors: | SU Jian-qing YUE Miao-shu WANG Hua-lei XUE Lin XIA Xian-zhu |
| Institution: | 1.College of Animal Science and Veterinary Medicine;Jilin University;Changchun 130062;China;2.Institute of Military Veterinary;Academy of Military Medical Sciences of PLA;3.College of Agriculture;Liaocheng University;Liaocheng 252059;4.College of Life Science and Technology;Changchun University of Science and Technology;China |
| Abstract: | Canine distemper virus was purified and used to immunize Balb/C mice.The hybridoma culture supernants were screened by indirect ELISA.The positive cells were subcloned until all clones were positive.The specificity of monoclonal antibodies was confirmed by Vero cells cross-reaction test and lFA.The Results showed that six hybridoma cells secreting anti-CDV McAb were obtained.These six cells were named AD7,AE2,CE3,EH5,GG6 and GF1.All the six McAbs confirmed by cross-reaction test and lFA were specificity aga... |
| Keywords: | canine distemper virus monoclonal antibodies enzyme-linked immunosorbent assay |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |