高效离子色谱法测定乳及乳制品中低聚果糖含量

建立离子色谱法定量测定乳及乳制品中低聚果糖的方法,应用CarboPacTMPA1(4mm×250mm)阴离子交换柱、氢氧化钠和醋酸钠淋洗液梯度淋洗、脉冲安培检测器检测。6次测定液态奶中低聚果糖的含量,相对标准偏差小于9%,3种糖的加标回收率在94.8%～103.6%之间。本方法简单快捷,适用于乳及乳制品中低聚果糖含量的检测。

Determination of Fructooligosaccharides in Milk and Milk Products by High Performance Ion Exchange Chromatography

An analytical method was established to determine fructooligosaccharides(FOS) in milk and milk products by high performance ion exchange chromatography method with pulsed amperometric detection.The column was a CarboPac PA1(4 mm × 250 mm) anion exchange column.Fructooligosaccharides were separated under gradient elution mode.The mobile phase A was 150 mmol/L NaOH and the mobile phased B was composed of 150 mmol/L NaOH and 100 mmol/L sodium acetate.The relative standard derivations for 6 replicate determinations of four fructooligosaccharides in liquid milk were less than 9%.The average spike recovery rates for three fructooligosaccharides in liquid milk varied between 94.8% and 103.6%.The method was simple,fast and suitable for determining FOS in milk and milk products.