4株Lactobacillus菌株对结肠癌细胞增生的抑制效果研究

以分离自传统发酵乳制品中的4株菌——Lactobacillus paracasei supsp.paracasei M5AL、L.paracasei supsp.paracasei J23ANL、L.paracasei supsp.paracasei G15AL、L.coryniformis supsp.torquens T3AL为实验菌株,采用噻唑兰法研究其热灭活菌体、细胞壁及DNA对结肠癌细胞HT-29增殖的影响。结果表明：菌株J23ANL、M5AL、G15AL、T3AL热灭活菌体细胞、菌体细胞壁和菌体DNA均可不同程度地抑制人结肠癌细胞HT-29的增生,其中菌株T3AL对HT-29细胞增生的抑制率显著高于其他菌株的抑制率（P〈0.05）;单细胞凝胶电泳结果显示菌株T3AL可诱导HT-29细胞凋亡。

Antiproliferative Effect of 4 Lactobacillus Strains on Colonic Cancer Cells

In this study,heat-killed bacterial cells,genomic DNA,and cell wall of 4 Lactobacillus strains isolated from traditional dairy food in China,Lactobacillus paracasei supsp.paracasei M5AL,L.paracasei supsp.paracasei J23ANL,L.paracasei supsp.paracasei G15AL,L.coryniformis supsp.torquens T3AL,were tested in vitro for their antiproliferative effect on colonic cancer cell line HT-29 by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay.All heat-killed bacterial cells,genomic DNA,and cell wall of these 4 strains exhibited direct antiproliferative activities against HT-29 cells.Lactobacillus coryniformis ssp.torquens T3AL exerted significantly stronger antiproliferative activities against HT-29 cells(P<0.05) than other three strains.The results of single cell gel electropherosis showed that the strain T3AL could induce apoptosis of HT-29 cells.Further study is needed to explain the mechanism of apoptosis of HT-29 cells induced by the strain.