百合品种离体培养的研究

对"布鲁拉诺"、"索蚌"、"提伯"3个百合品种的离体培养进行了比较研究.结果表明:接种百合鳞片外植体的最佳消毒方法是0.1%升汞消毒8 min,污染率较低,为11.0%,而成活率最高,可达88.0%;采用小鳞茎整体消毒可以有效降低污染率,污染率为0.0%;不同百合品种分化小鳞茎的能力有较大差异,分化能力依次为"布鲁拉诺"＞"索蚌"＞"提伯";在继代增殖培养中,"布鲁拉诺"的增殖系数最高,迭5.0,其次为"索蚌","提伯"最低,为4.2;3个百合品种的试管苗生根率都较高,其中"布鲁拉诺"最高,为92.0%,"索蚌"87.5%,"提伯"83.3%.

Study on Tissue Culture of Lily Cultivars

The bulb scales of three lily cultivars 'Brunello','Tiber' and 'Sorbonne' were used as explants to culture in vitro to compare their difference in the experiment.The results showed that the best sterilization method was 0.1% HgCl2 with 8 minutes.The rate of pollution was lower with only 11.0% and the rate of survival was up to 88.0%.The rate of pollution could be effectively reduced using the sterilization method for the whole bulb with the pollution rate of almost 0.0%.The capability of differentiating bulblets from the callus for different lily cultivars was various obviously,and from strong to weak was 'Brunello','Sorbonne',and then 'Tiber' in the experiment.'Brunello' had the highest propagation coefficient and reached 5.0,and the next was 'Sorbone',and 'Tiber' was the lowest with the propagation coefficient of 4.2.The rate of rooting of three lily cultivars,'Brunello','Sorbonne',and 'Tiber' was high with 92.0%,87.5% and 83.30% respectively.