鱼腥草中槲皮素含量分析方法研究

[目的]建立高效液相色谱法测定鱼腥草不同部位中天然槲皮素含量的方法。[方法]利用纤维素酶与超声提取相结合;采用色谱柱为Eclipse XDB-C18（4.6 mm×150 mm,5μm）,流动相为乙腈-1%冰乙酸（40∶60）,柱温为25℃,流速为0.8 ml/min,检测波长为370nm。[结果]槲皮素在0.1~14.4μg/ml范围内呈良好线性关系（r=0.999 9）,鱼腥草根样品的平均回收率为99.2%,RSD为3.9%,茎叶样品的平均回收率为98.8%,RSD为3.8%。[结论]所建立的方法分离效果好,专属性强,操作简便,结果准确,重现性好,适用于鱼腥草中槲皮素含量的测定。

Study on the Analysis Methods of Quercetin Content in Houttuynia cordata Thunb

[Objective]The aim was to establish a HPLC method for the content determination of natural quercetin in different parts of Houttuynia cordata Thunb.[Methods] The combination between the cellulase and ultrasonic extracting was used;and the chromatographic column was XDB-C18(4.6 mm×150 mm,5 μm),mobile phase was acetonitrile-1% acetic acid(40∶60),column temperature was 25 ℃,and the flow velocity was 0.8 ml/min,the detection wavelength was 370 nm.[Result] There was good linear relationship for linear within the range of 0.1-14.4 μg/ml(r=0.999 9) and the average recovery of quercetin in the roots of Houttuynia cordata Thunb samples was 99.2% and RSD was 3.9%.The average recovery of quercetin in the stems and leaves of Houttuynia cordata Thunb was 98.8% and RSD was 3.8%.[Conclusion]The established methods had many advantages,such as effective separating effect,speciality,simple operation,accurate results and good reproducibility,therefore,it can be applied for the determination of quercetin in Houttuynia cordata Thunb.