酒糟菌糠水溶性多糖的提取与含量测定

目的]探讨了酒糟菌糠多糖提取的最佳工艺条件及测定4种酒糟菌糠多糖的含量,为酒糟菌糠的进一步开发提供依据。方法]以茶树菇菌糠（未出菇）为材料,采用正交试验,进行水提醇沉法优选。根据优选出的最佳工艺条件,对茶树菇菌糠（出菇）、茶树菇菌糠（未出菇）、鸡腿菇菌糠（出菇）和鸡腿菇菌糠（未出菇）这4种酒糟菌糠进行粗多糖的提取,并采用硫酸-苯酚比色法测定粗多糖中总糖含量和DNS法测定粗多糖中还原糖的含量。结果]酒糟菌糠粗多糖的最佳提取条件为料液比1∶30,提取温度90℃,提取时间4 h;测得上述4种酒糟菌糠中多糖含量分别为0.39%、0.70%、0.47%、0.99%。结论]未出菇的酒糟菌糠多糖含量高于出菇的酒糟菌糠,培养鸡腿菇的酒糟菌糠多糖含量高于培养茶树菇的酒糟菌糠。

Extraction and Determination of Water-soluble Polysaccharides from Distiller's Grains-based Spent Mushroom Substrate

Objective] The extraction technology of distiller′s grains-based spent mushroom substrate(SMS) and the content of polysaccharide of four kinds of distiller′s grains-based on SMS were studied so as to provide the basis for the further exploration of distiller′s grains-based SMS.Method] Taking Agrocybe aegerita substrate(not produced mushroom) as the material,the method of water extracting-alcohol precipitating was optimized through orthogonal test.According to the optimal extracting technological conditions,the yield of crude polysaccharide of Agrocybe aegerita substrate(produced mushroom),Agrocybe aegerita substrate(not produced mushroom),Coprinus comatus substrate(produced mushroom) and Coprinus comatus substrate(not produced mushroom) were determined.Phenol-Sulphate acid method was used to detect the content of total sugar in crude polysaccharide.DNS method was used to detect the content of reducing sugar in crude polysaccharide.Result] The optimal extracting technological conditions were as follows:the liquid ratio was 1∶30,the extraction temperature was 90℃ and the extraction time was 4 hours.The content of polysaccharide of these four kinds of distiller′s grains-based SMS respectively was 0.39%,0.70%,0.47% and 0.99%.Conclusion] The yield of polysaccharide of distiller′s grains-based SMS(not produced mushroom) was higher than distiller's grains-based SMS(produced mushroom).The content of polysaccharide of Coprinus comatus substrate was higher than that of Agrocybe aegerita substrate.