| 超高压液相色谱法测定金银花中绿原酸的含量 |
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| 引用本文： | 卢建锋. 超高压液相色谱法测定金银花中绿原酸的含量[J]. 安徽农业科学, 2011, 39(7): 3946-3947 |
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| 作者姓名： | 卢建锋 |
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| 作者单位： | 漯河医学高等专科学校,河南,漯河,462002 |
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| 摘 要： | [目的]建立金银花中绿原酸含量的UPLC测定方法。[方法]采用ACQUITY UPLCTM BEHC C18柱,乙腈：0.1%冰醋酸为流动相进行梯度洗脱。[结果]绿原酸含量在2.0-10.0μg/L浓度范围内呈良好的线性关系,回收率为99.8%,RSD为1.21%。[结论]该法前处理简单、分析速度快、重复性好、检出限低,可作为金银花中绿原酸含量的测定方法。
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| 关 键 词： | 超高压液相色谱 金银花 绿原酸 |
Content Determination of Chiorogenic Acid In Flos Lonicerae By Ultra Performance Liquid Chromatography(UPLC) |
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| LU Jian-feng. Content Determination of Chiorogenic Acid In Flos Lonicerae By Ultra Performance Liquid Chromatography(UPLC)[J]. Journal of Anhui Agricultural Sciences, 2011, 39(7): 3946-3947 |
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| Authors: | LU Jian-feng |
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| Affiliation: | LU Jian-feng(Luohe Medical College,Luohe,Henan 462002) |
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| Abstract: | [Objective]This paper aims to establish a method for content determination of chlorogenic acid in Flos Lonicerae by UPLC.[Methods]Chlorogenic acid analysis in Flos Lonicerae is performed on an ACQUITY UPLCⅢ BEHC C18 columnby a gradient elution employing of acetonitrile-0.1% acetic acid as a mobile phase.[Results]This methods has good linearity in the range of 2.0-10.0 μg/L.The average recovery is 99.8%.RSD is 1.21%.[Conclusion]It indicates that the method is simple,sensitive and accurate with a good reproducibility,which can meet the demand for the determining chlorogenic acid analysis in Flos Lonicerae. |
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| Keywords: | UPLC Flos Lonicerae Chlorogenic acid |
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