车前草药材的质量标准研究

目的]对车前草药材质量标准进行研究。方法]采用薄层色谱法对车前草进行定性鉴别,展开剂为乙酸乙酯-石油醚（30~60℃）-甲酸（10∶15∶1,V/V）,显色剂为1%氯化铝乙醇溶液;采用HPLC法测定车前草药材中木犀草素的含量,色谱柱为Alltech Apollo C18（4.6 mm×250.0 mm,5μm）,流动相为甲醇-0.2%磷酸溶液（55∶45,V/V）,流速为1.0 ml/min,检测波长为349 nm,柱温为35℃。结果]薄层色谱鉴别中,供试品色谱在与对照品、对照药材色谱相应的位置上,显示相同颜色的荧光斑点。木犀草素在1.05~21.00μg/ml浓度范围内与峰面积呈良好的线性关系（r=0.999 8）,方法平均回收率为99.60%,RSD值为1.70%。结论]该方法准确、可靠、灵敏度高、专属性强,可有效控制车前草药材的质量。

Study on Quality Standard for HERBA PLANTAGINIS

Objective] To supplement the quality standard for HERBA PLANTAGINIS.Method]TLC was used to qualitatively identify the main ingredients using ethylacetate-petroleum ether(30-60 ℃)-formic acid(V/V,10∶15∶1) as a developing agent.HPLC was used to quantitatively determine the luteolin content using Alltech Apollo C18(4.6 mm×250.0 mm,5 μm)column as chromatographic column and acetonitrile-0.2% phosphoric acid solution(V/V,55∶45) as the mobile phase at a flow rate of 1.0 ml/min.The detection wavelength was 349 nm and the column temperature was 35 ℃.Results]On the same plate of TLC,the reference and control medicinal material were on the same position corresponding to particular color in the spectrum.The calibration curve was linear in the range of 1.05-21.00 μg/ml(r=0.999 8) for luteolin.The average recovery rate of luteolin were 99.60%(RSD=1.70%).Conclusion] The method established in this study is reliable,accurate and specific,which can be used for the quality control of HERBA PLANTAGINIS.