| 猪链球菌2型溶血素融合蛋白的制备及免疫活性测定 |
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| 引用本文： | 王海丽,徐公义,葛长城,赵德明. 猪链球菌2型溶血素融合蛋白的制备及免疫活性测定[J]. 安徽农业科学, 2011, 39(6): 3604-3605,3614 |
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| 作者姓名： | 王海丽 徐公义 葛长城 赵德明 |
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| 作者单位： | 1. 中国农业大学动物医学院,北京,100094;聊城职业技术学院,山东聊城,252000
2. 中国农业大学动物医学院,北京,100094 |
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| 基金项目： | 山东省教育厅科技计划项目，2010年度高等学校优秀青年教师国内访问学者项目，山东省聊城市科技攻关计划项目 |
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| 摘 要： | [目的]研究四川资阳脑膜炎病例猪链球菌2型分离株ZYH33溶血素（suliysin,sly）基因的克隆表达及融合蛋白生物活性分析。[方法]从sly中获取第230~593氨基酸残基区域的基因片段,克隆至pMD18-T载体并鉴定,以重组pMD18-T/sly为模板PCR扩增基因片段,与表达载体pQE-30连接,转化至大肠杆菌TG1,重组子经PCR、酶切、测序鉴定。IPTG诱导重组蛋白表达,Western blot法鉴定融合蛋白的抗原性。[结果]基因片段在大肠杆菌中得到了表达,表达的融合蛋白可被猪链球菌2型菌体ZY05719抗血清识别,具有抗原性。[结论]所克隆、表达的溶血素区域可作为猪链球菌的诊断抗原,为基因工程疫苗的研制奠定了基础。
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| 关 键 词： | 猪链球菌2型 溶血素 克隆 表达 抗原性 |
Preparation and Immunological Activity Analysis for the Gene Engineering Streptococcus suis Serotype 2 Suilysin Fusion Protein |
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| WANG Hai-li et al. Preparation and Immunological Activity Analysis for the Gene Engineering Streptococcus suis Serotype 2 Suilysin Fusion Protein[J]. Journal of Anhui Agricultural Sciences, 2011, 39(6): 3604-3605,3614 |
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| Authors: | WANG Hai-li et al |
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| Affiliation: | WANG Hai-li et al(China Agricultural University,Beijing 100094) |
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| Abstract: | [Objective] To study human Streptococcus suis serotype 2 suilysin fusion protein and investigate its immunological activity.[Method] sly gene was obtained from ZYH33,which was isolated from human meningitis in Ziyang of Sichuan province.The gene was amplified by PCR with the template pMD18-T/sly.The gene fragments and expressing vector pQE-30 were ligated.The recombinant vector were transformed into TG1.The correct recombinations were identified by PCR,enzyme digestion and sequencing.Recombination protein a... |
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| Keywords: | Streptococcus suis serotype 2 Suilysin Clone Expression Antigenicity |
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