可见分光光度法测定胡椒碱纯度的研究 |
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引用本文: | 吴琼,谭娟娟,刘业明,史载锋. 可见分光光度法测定胡椒碱纯度的研究[J]. 安徽农业科学, 2011, 39(7): 3973-3974,3980 |
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作者姓名: | 吴琼 谭娟娟 刘业明 史载锋 |
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作者单位: | 海南师范大学化学与化工学院,海南,海口,571158 |
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摘 要: | [目的]考察可见分光光度法测定胡椒碱含量的最佳溶剂和最适宜检测波长。[方法]以二氯乙烷、乙酸乙酯和无水乙醇作为溶剂,考察胡椒碱在380~420 nm波长范围内的最佳溶剂和测定波长,并将其测定纯度与HPLC法进行比较。[结果]最佳的溶剂为无水乙醇;在较低浓度范围内(0~0.01 g/L),胡椒碱最适宜的测定波长为380 nm,而在较高浓度范围(0.20~1.00 g/L),其最适宜的测定波长则为400 nm。可见分光光度法所测定的样品中胡椒碱纯度与HPLC法进行比较,平均误差为0.85%。[结论]利用可见分光光度计测定胡椒碱纯度具有较高的准确度,是可行的。
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关 键 词: | 胡椒碱 纯度 可见分光光度法 |
Study on Purity of Piperine by UV-VIS Spectrophotometry |
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Affiliation: | WU Qiong et al(School of Chemistry and Chemical Engineering,Hainan Normal University,Haikou,Hainan 571158) |
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Abstract: | [Objective] To investigate the optimal solvent and detection wavelength for piperine by UV-VIS spectrophotometry.[Method] Using dichloroethane,ethyl acetate and absolute ethanol as solvent,the optimal solvent and detection wavelength of piperine were surveyed at wavelength of 380-420 nm,and the determined result was compared with that detected by HPLC.[Result]The optimum solvent was absolute ethanol,and the optimum detection wavelength for piperine was 380 nm at lower concentration(0-0.01 g/L),while that was 400 nm at higher concentration(0.20-1.00 g/L).The mean error between the purity determined by UV-VIS spectrophotometry and HPLC method was just 0.85%.[Conclusion] Good in precision,UV-VIS spectrophotometry is favorable for the determination of piperine. |
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Keywords: | Piperine Purity UV-VIS spectrophotometry |
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