降低软籽石榴组织培养过程中外植体褐化的研究

目的]研究基本培养基、植物生长物质种类和浓度及防褐剂含量对石榴组织培养外植体褐化的影响。方法]选用淮北黄里软籽石榴的茎段为材料,采用正交试验研究基本培养基、6苄-基腺嘌呤（6-BA）、吲哚乙酸（IAA）、聚乙烯吡咯烷酮（PVP）对外植体褐化的影响,对所得试验数据进行极差分析和方差分析,探讨软籽石榴茎段组织培养褐化率最低的培养基。结果]淮北黄里软籽石榴茎段组织培养褐化率最低的培养基为MS＋6-BA1.0 mg/L＋IAA 0.50mg/L＋PVP 2500mg/L＋蔗糖30 g/L＋琼脂7g/L,各因素对褐化率的影响程度依次为PVP〉6-BA〉无机盐〉IAA。结论]该研究可为进一步提高软籽石榴快速繁殖系数提供依据。

Study on Decreasing Browning of Explants in Tissue Culture of Soft Seeding Pomegranate

Objective] To study the effects of different factors on explants browning,including basic culture medium,plant growth substances and antioxidant browning substances.Method] Choosing the stem segment of Huaibei soft seeding pomegranate as material in the experiment to study the influences of basic culture medium,6-bian base adenine(6-BA),indole acetic acid(IAA) and polyvinylpyrrolidone(PVP) through orthogonal test;meanwhile,range analysis and variance analysis were utilized by the experimental data,the minimum browning rate culture medium for culturing soft seeding pomegranate was discussed.Result] The optimal media for reducing browning rate of the stem segment was MS + 6-BA 1.0 mg/L + IAA 0.50 mg/L + PVP 2 500 mg/L +sucrose 30 g/L + agar 7 g/L,the effect of the factors was in sequence of PVP>6-BA>inorganic salts>IAA.Conclusion] The study can provide the basis for developing rapid propagation ratio of soft seeding pomegranate.