| 利用巢式PCR方法筛选水稻OsIRT1突变体的研究 |
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| 引用本文： | 王俐勇,苏红,印莉萍. 利用巢式PCR方法筛选水稻OsIRT1突变体的研究[J]. 安徽农业科学, 2011, 39(6): 3176-3178,3199 |
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| 作者姓名： | 王俐勇 苏红 印莉萍 |
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| 作者单位： | 首都师范大学,北京,100048;首都师范大学,北京,100048;首都师范大学,北京,100048 |
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| 摘 要： | [目的]筛选得到水稻OsIRT1基因突变体。[方法]采用巢式PCR方法从水稻Tos17插入突变体库中筛选,共筛选近30000个单株。[结果]得到一个OsIRT1突变体单株。[结论]该突变体中的Tos17插入位点位于OsIRT1的内含子中。
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| 关 键 词： | 水稻 突变体 OsIRT1 巢式PCR |
Rice OsIRT1 Mutant Screening by Nest PCR |
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| Affiliation: | WANG Li-yong et al(Capital Normal University,Beijing 100048) |
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| Abstract: | [Objective] The research aimed to screen OsIRT1 mutant of rice.[Method] Nested PCR was adopted to screen from Tos17 insertion mutant library of rice,in which nearly thirty thousand lines were selected.[Result] One OsIRT1 mutant was confirmed.[Conclusion] Mutation of Tos17 insertion site was located in intron OsIRT1. |
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| Keywords: | OsIRT1 |
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