鳜鱼嗜水气单胞菌的分离与鉴定

在建立和完善北方地区鱼类疫病的流行病学调查研究时,从丹东鸭绿江一网箱养殖场患病鳜鱼中分离到了一株优势菌株。对该菌株进行营养琼脂及绵羊血琼脂培养基培养、革兰氏染色镜检、Vitek细菌鉴定仪鉴定及16SrRNA序列分析比对。经细菌形态学特征分析、生理生化试验及16SrRNA核苷酸序列同源性分析和系统发育树的构建比对,结果表明该分离菌株为嗜水气单胞菌。本研究的开展为有效防控疫病及疫苗制备等方面提供参考和借鉴,同时为开展该疫病的监测、防治和预警,促进鱼类及其产品的进出口贸易,意义十分重大。

Isolation and Identification of Aeromonas hydrophila in Sinipercachuatsi

A pathogenic bacterium was isolated from the infected Sinipercachuatsi in aquaculture farms in Dandong in the study of epidemiology investigation on fish diseases in northern area and identified by nutrient agar medium,blood agar medium,Gram staining test,Vitek Microbiology Identification System,physiological biochemical test and 16S rRNA sequences alignments and phylogenetic tree as Aeromonas hydrophila.The research provided support to disease prevention control and vaccine preparation for Sinipercachuatsi,and it was very important for quarantine inspection and international trade of fish and fish products.