| 甘蔗花叶病毒河南分离物HC-Pro基因的序列分析及其RNA沉默表达载体构建 |
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| 引用本文： | 夏宗良,武轲,周朋,吴建宇. 甘蔗花叶病毒河南分离物HC-Pro基因的序列分析及其RNA沉默表达载体构建[J]. 河南农业大学学报, 2010, 44(2) |
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| 作者姓名： | 夏宗良 武轲 周朋 吴建宇 |
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| 作者单位： | 河南农业大学生命科学学院,河南,郑州,450002;河南农业大学生命科学学院,河南,郑州,450002;河南农业大学生命科学学院,河南,郑州,450002;河南农业大学生命科学学院,河南,郑州,450002 |
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| 基金项目： | 河南省科技攻关重点项目 |
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| 摘 要： | 采用RT-PCR方法从河南地区甘蔗花叶病毒(Sugarcane mosaic virus,SCMV)感染的玉米叶片中,克隆了辅助成分-蛋白酶基因(helper component.proteinase,HC-Pro).该基因长1 380 bp,编码460个氨基酸.序列比对表明.HC-Pro编码的氨基酸序列与北京、山东地区SCMV分离物的相似性分别达到97%和98%.而与以前数据库登录的河南分离物的相似性仅98%,说明河南地区SCMV可能发生了变异.选取长582 bp的HC-Pro片段,构建了表达发夹RNA的基因沉默双元表达载体pRNAi-HC-Pro(±),成功转化了农杆菌LBA4404.
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| 关 键 词： | 甘蔗花叶病毒 玉米 辅助成分-蛋白酶 RNA干扰 |
Sequence analysis and construction of RNA silencing expression vector of the Hc-Pro gene from Sugarcane mosaic virus-Henan isolate |
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| XIA Zong-liang,WU Ke,ZHOU Peng,WU Jian-yu. Sequence analysis and construction of RNA silencing expression vector of the Hc-Pro gene from Sugarcane mosaic virus-Henan isolate[J]. Journal of Henan Agricultural University, 2010, 44(2) |
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| Authors: | XIA Zong-liang WU Ke ZHOU Peng WU Jian-yu |
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| Affiliation: | College of Life Sciences/a>;Henan Agricultural University/a>;Zhengzhou 450002/a>;China |
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| Abstract: | The complete helper component-proteinase(HC-Pro) gene was cloned by RT-PCR from diseased maize leaves infected by Henan isolate of Sugarcane mosaic virus(SCMV).The cDNA of the HC-Pro contains a 1 380 bp open reading frame(ORF) and encodes 460 amino acids.Sequence alignment showed that the deduced amino acid sequence of the HC-Pro shared 97% and 98% similarities to homologues from SCMV Beijing isolate and Shandong isolate respectively.However,the Hc-Pro cloned by our group only shared 98% similarity to that ... |
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| Keywords: | Sugarcane mosaic virus maize helper component-proteinase RNA interference |
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