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Comparison of two versions of larval development test to detect anthelmintic resistance in Haemonchus contortus |
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| Authors: | Marin Vrady Július orba Valria Letkov Gabriel Kov |
| Institution: | aParasitological Institute of the Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovak Republic;bUniversity of Veterinary Medicine, Department of Epizootology and Parasitology, Komenského 73, 041 81 Košice, Slovak Republic;cUniversity of Veterinary Medicine, Clinic for Ruminants, Komenského 73, 041 81 Košice, Slovak Republic |
| Abstract: | Larval development (LDT) and micro-agar larval development tests (MALDT) were used to compare the reliability and sensitivity of two methods for detecting anthelmintic resistance in Haemonchus contortus. The tests were conducted using three resistant and four susceptible isolates of H. contortus. Both versions of the tests provided comparable results with regard to the characterization of benzimidazole and levamisole susceptibility but neither test was sufficiently sensitive to discrimination between an ivermectin (IVM) susceptible and an IVM resistant isolate. Each test has its own merits with the LDT having the advantage of being less time-consuming. |
| Keywords: | Anthelmintic resistance In vitro detection Haemonchus contortus Benzimidazole Ivermectin |
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