| Construction of Recombinant Expression Plasmid pYELmleA of mleA Gene and Expression in Saccharomyces cerevisiae |
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| 引用本文： | 刘延琳,蒋思欣,何秀萍,李 华,张博润. Construction of Recombinant Expression Plasmid pYELmleA of mleA Gene and Expression in Saccharomyces cerevisiae[J]. 中国农学通报, 2005, 21(4): 48-48 |
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| 作者姓名： | 刘延琳 蒋思欣 何秀萍 李 华 张博润 |
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| 作者单位： | 1. 西北农林科技大学葡萄酒学院,陕西,杨凌,712100
2. 中国科学院微生物研究所,北京,100080 |
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| 基金项目： | 陕西省自然基金及西北农林科技大学科研专项资助. |
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| 摘 要： | 苹果酸－乳酸酶是进行MLF的功能酶。笔者进行酒酒球菌SD-2a的苹果酸－乳酸酶基因重组表达质粒的构建，利用来自重组质粒pLmleA的mleA基因，以PGK1强启动子和ADH1终止子为调控元件，以大肠杆菌－酵母菌穿梭质粒YEp352为载体，构建了重组表达质粒pYELmleA，并转化酿酒酵母（Saccharomyces cerevisiae）YS58。酵母转化子用含有亮氨酸、组氨酸和色氨酸的YNB平板筛选鉴定。SDS-PAGE检测表明获得的转化子表达了约60KD的目标蛋白，斑点杂交检测表明目的基因mleA转化到
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| 关 键 词： | 酒酒球菌 苹果酸－乳酸酶基因 重组表达质粒 酿酒酵母 |
Construction of Recombinant Expression Plasmid pYELmleA of mleA Gene and Expression in Saccharomyces cerevisiae |
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| Liu Yanlin,Jiang Sixin,He Xiuping,Li Hu,Zhang Borun. Construction of Recombinant Expression Plasmid pYELmleA of mleA Gene and Expression in Saccharomyces cerevisiae[J]. Chinese Agricultural Science Bulletin, 2005, 21(4): 48-48 |
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| Authors: | Liu Yanlin Jiang Sixin He Xiuping Li Hu Zhang Borun |
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| Affiliation: | (1College of Enology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100)（ 2Institute of Microbiology，Academia Sinica，Beijing 100080） |
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| Abstract: | Malolactic enzyme is the function enzyme to turn L-malic acid into L-lactic acid during malolactic fermentation (MLF). In this paper, mleA gene encoding for malolactic enzyme from pLmleA, PGK1 promoter from plasmid pVC727-6 and ADH1 terminator from plasmid |
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| Keywords: | Oenococcus oenizz Malo-lactic enzyme genezz Recombinant expression Plasmidzz Saccharomyces cerevisiaezz |
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