| 上海市猪圆环病毒2型全基因组的克隆与序列分析 |
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| 引用本文： | 刘健,鞠厚斌,张维谊,杨德全,王建,刘佩红,周锦萍. 上海市猪圆环病毒2型全基因组的克隆与序列分析[J]. 中国动物传染病学报, 2012, 0(3): 73-77 |
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| 作者姓名： | 刘健 鞠厚斌 张维谊 杨德全 王建 刘佩红 周锦萍 |
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| 作者单位： | 上海市动物疫病预防控制中心 |
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| 摘 要： | 参照国外发表的猪圆环病毒2型(Porcine circovirus type 2,PCV-2)全基因组序列,设计合成了1对特异性引物,从上海市临床病料中提取PCV-2基因组DNA,进行PCR全基因扩增。回收PCR产物,将其插入pMD18-T载体,并对筛选出的阳性质粒进行测序。应用DNAStar序列分析软件,对所测PCV-2序列与GenBank中登录的国内外PCV-2毒株进行同源性比较。结果显示,PCV-2上海株与国内外毒株的核苷酸同源性高达95.0%～100%。进化树分析结果显示,其中9株PCV-2病毒属于PCV-2b亚型,3株PCV-2病毒属于PCV-2a亚型,且9株PCV-2b亚型毒株部分核苷酸位点显示其属于强毒力毒株。研究表明,上海市PCV-2感染以PCV-2b亚型为主,且氨基酸位点表明其具有较高的毒力。
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| 关 键 词： | 猪圆环病毒2型 基因组 序列分析 |
CLONING AND SEQUENCE ANALYSIS OF THE COMPLETE GENOMES OF 12 SHANGHAI STRAINS OF PORCINE CIRCOVIRUS TYPE 2 |
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| LIU Jian,JU Hou-bin,ZHANG Wei-yi,YANG De-quan,WANG Jian,LIU Pei-hong,ZHOU Jin-ping. CLONING AND SEQUENCE ANALYSIS OF THE COMPLETE GENOMES OF 12 SHANGHAI STRAINS OF PORCINE CIRCOVIRUS TYPE 2[J]. Chinese Journal of Animal Infectious Diseases, 2012, 0(3): 73-77 |
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| Authors: | LIU Jian JU Hou-bin ZHANG Wei-yi YANG De-quan WANG Jian LIU Pei-hong ZHOU Jin-ping |
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| Affiliation: | (Shanghai Animal Disease Control Center,Shanghai 201103,China) |
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| Abstract: | According to the published genome sequences of PCV-2,a pair of specific primers was designed to amplify the full length genome from clinical pig tissues collected from Shanghai area.The obtained fragments were cloned into pMD18-T vector for sequence analysis.These 12 PCV-2 Shanghai strains shared 95.0%~ 100% nucleotide identities with other PCV-2 strains in GenBank.Phylogenetic analysis showed that 9 Shanghai strains belonged to PCV-2b subtype and 3 strains to PCV-2a subtype.Variations at some amino acid positions suggested high virulence of 9 PCV-2b strains. |
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| Keywords: | Porcine circovirus type 2(PCV-2) genome sequence analysis |
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