| 水稻种胚脂肪氧化酶Lox1基因的遗传分析及定位 |
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| 引用本文： | 蒋家月,吴跃进,张从合,蒋家平,刘斌美.水稻种胚脂肪氧化酶Lox1基因的遗传分析及定位[J].核农学报,2012(4):629-633. |
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| 作者姓名： | 蒋家月 吴跃进 张从合 蒋家平 刘斌美 |
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| 作者单位： | 安徽农业大学安徽省食品安全分析与检测实验室;中科院等离子体物理研究所离子束生物工程学重点实验室;安徽荃银高科种业股份有限公司 |
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| 基金项目： | 中国科学院知识创新工程重要方向项目(KSCX2SW2324);安徽省高校省级科研项目(KJ2012A120);安徽农业大学人才基金(yj2009-03) |
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| 摘 要： | 为研究水稻种胚脂肪氧化酶Lox1的遗传规律及分子机制,以Lox1缺失突变体1297分别与Lox1活性正常的9311、日本晴杂交,构建2个F2群体,对2个F2群体种子的种胚Lox1进行定量测定和遗传学分析,结果表明低Lox1是受1对单基因控制的隐性性状。以1297与日本晴组配的F2分离群体300个单株为定位群体,将水稻种胚低Lox1基因定位于水稻第3染色体的RM4512和RM282之间,距两侧标记的遗传距离分别为13.0cM和9.1cM,为进一步的分子标记辅助育种和图位克隆打下了基础。通过人工加速老化对种子进行了耐储性评价,表明水稻种胚Lox1与种子储藏特性关系密切。
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| 关 键 词： | 水稻 脂肪氧化酶Lox1 遗传分析 基因定位 |
GENETIC ANALYSIS AND MAPPING OF LIPOXYGENASE ISOENZYME Lox1 OF RICE EMBRYO |
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| Institution: | JIANG Jia-yue1,2 WU Yue-jin2 ZHANG Cong-he3 JIANG Jia-ping3 LIU Bing-mei2(1.Key Laboratory of Analysis and Detection Technology for Food Safety,Anhui Agricultural University,Hefei,Anhui 230036; 2.Key Laboratory of Ion Beam Bioengineering,Institute of Plasma Physics,Chinese Academy of Sciences,Hefei,Anhui 230031; 3.Anhui Win-all High-Tech Seed Co,Ltd.Hefei,Anhui 230088) |
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| Abstract: | In order to study the inheritance and genetic mechanism of Lox1 in rice embryos,1297(lack of Lox1) was crossed with cv.9311(normal) and cv.Nipponbare(normal),respectively.The results showed that low Lox1 activity was a recessive trait controlled by a pair of recessive gene.The mapping population consisting of 300 F2 lines from across between cv.1297 and cv.Nipponbare was used to identify a null-locus for Lox1.Extreme high or low Lox1 activity DNA pools were established to screen the SSR markers which were distinct in both parents and DNA pools.The gene Lox1 was mapped between RM4512 and RM282 on the chromosome 3 based on SSR analysis,with genetic distances apart from the flanking markers of 13.0cM and 9.1cM,respectively.These results are useful for further cloning and functional analysis of the Lox1 gene.In addition,storage characteristics of F2 population were assayed.The storage characteristic was significantly correlated with the Lox1 activity. |
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| Keywords: | rice(Oryza sativa L ) lipoxygenase isoenzyme Lox1 genetic analysis gene mapping |
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