| 一个新的大豆类受体蛋白激酶基因rlpk2的克隆及其结构特征 |
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| 引用本文： | 李小平,甘睿,马媛媛,李鹏丽,张丽文,王勇,张韧,王宁宁. 一个新的大豆类受体蛋白激酶基因rlpk2的克隆及其结构特征[J]. 大豆科学, 2005, 24(4): 249-255 |
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| 作者姓名： | 李小平 甘睿 马媛媛 李鹏丽 张丽文 王勇 张韧 王宁宁 |
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| 作者单位： | 南开大学植物学及生态学系,天津,300071;Department of Biological Sciences,University of Wollongong, NSW2522, Australia |
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| 摘 要： | 植物类受体蛋白激酶在细胞生长及对环境胁迫反应方面起着非常重要的作用.本文报告用RACE方法(快速扩增cDNA末端)扩增一个新的大豆类受体蛋白激酶基因rlpk2的全长cDNA.根据RACE的结果,我们克隆了rlpk2的cDNA,其序列信息已经提交到GenBank(Accession No.AY687391).经初步分析,rlpk2编码产物为LRR型类受体蛋白激酶.
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| 关 键 词： | 大豆 末端转移酶 类受体蛋白激酶 快速扩增cDNA末端 |
| 文章编号： | 1000-9841（2005）04-0249-07 |
| 收稿时间： | 2005-01-21 |
| 修稿时间： | 2005-01-21 |
LONE AND CHARACTERISTICS OF A NOVEL SOYBEAN RECEPTOR-LIKE PROTEIN KINASE rlpk 2 GENE |
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| Li Xiaoping,Gan Rui,Ma Yuanyuan,Li Pengli,Zhang Liweng,Wang Yong,Zhang Ren,Wang Ningning. LONE AND CHARACTERISTICS OF A NOVEL SOYBEAN RECEPTOR-LIKE PROTEIN KINASE rlpk 2 GENE[J]. Soybean Science, 2005, 24(4): 249-255 |
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| Authors: | Li Xiaoping Gan Rui Ma Yuanyuan Li Pengli Zhang Liweng Wang Yong Zhang Ren Wang Ningning |
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| Affiliation: | 1. Nan Kai University Plant Biology and Ecology Department Tianjin 300071; 2. Department of Biological Sciences, University of Wollongong, NSW2522, Australia |
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| Abstract: | Receptor-like protein kinases (RLKs) play major roles in plant cellular processes and stress responses. Here we reported rapid amplification of cDNA ends ( RACE)of a novel soybean receptor-like kinase gene rlpk 2. Full length rlpk 2 cDNA was cloned according to the results of RACE. Details of PCR condition were discussed. The resultant cDNA sequence has been submitted into the GenBank database and assigned the accession No. AY687391. |
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| Keywords: | Soybean Terminal deoxynucleotidyl Transferase (TdT) Receptor-like protein kinase Rapid Amplification of a cDNA Ends (RACE) |
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