秃杉的组织培养*

Tissue Culture of Taiwania flousiana Gaussen

The embryo of imbibitional seed, the apex of 10~12- or 20-day-old seedling (which included cotyledons, shoot apex and 2~3mm hyp-ocotyl stub) were used as explants to induce the adventitious buds. The result showed that the percentage of the induced adventitious buds of the 3 explants were 31.4 %, 80 % and 0 respectively. The proliferation of adventitious bud was firstly depend on the developmental stage of the explant, and secondly on the concentration of N6-benzyl adenine (BA). The optimum concentration of BA for the induction of adventitious bud was 2.0mg/L. The elongated shoots were incubated in 1/2 Murashige and Skoog (MS) medium supplemented with 1.0~2.0mg/L IBA to inducee rooting, 80 % of the shoots were rooted. The residual bases of the shoots were transferred to MS medium in the presence of 1.0~2.0mg/L BA, and 3~5 adventitious buds were regenerated in each base.