新疆海岛棉的丛生芽诱导和茎尖遗传转化的研究

 以新疆海岛棉新海17号、新海14号、85H为材料，对海岛棉器官发生再生体系和遗传转化进行研究。结果表明：茎尖系统再生能力强，茎尖组培成苗率可达90.5%。在MSB5培养基中，6-BA浓度为0.5 mg ·L^-1时，芽诱导率最高；但每个外植体的出芽数所需的最佳浓度是1.5 mg·L^-1；当浓度为2.0 mg ·L^-1时，开始有抑制出芽的现象；每个外植体最多能诱导4个芽。用农杆菌介导法转化棉花的茎尖，对侵染的损伤和Kan的耐受能力强，对Kan棉花的茎尖选择压达100 mg· L^-1；抗性绿苗率可达88.9%。

Multiple-shoot Induction and Genetic Transformation of Island Cotton(Gossypium barbadense L.) in Xinjiang

With three island cottons, Xinhai 17, Xinhai 14, 85H, shoot organogenesis and genetic transformation were studied in Xinjiang.The results were as follows: in vitro plant regeneration of meristem shoot tip is better, and the seedling rate of 90.5%was obtained.Frequency of inducing shoots reached maximum with 6-BA at 0.5 mg·L-1; whereas number of budding shoots in per explant reached maximum with 6-BA at 1.5 mg·L-1; plantlet from shoot apical meristem repressed with 6-BA at 2.0 mg·L-1;The maximum budding shoots in per explant is four.Isolated meristem shoot tips infected with Agrobacterium tumefacien have strong endurance on infected damnification and kan, of which selective concentration reached 100 mg·L-1.The rate of plantlet from infected shoot tips on selection medium reached to 88.9%.