根癌农杆菌介导的马铃薯高效遗传转化体系的研究

通过根癌农杆菌介导的遗传转化方法,建立了马铃薯栽培品种“费乌瑞它(Favorita)”茎段和试管薯的遗传转化体系,其转化频率分别可达25.6%和36.8%。实验结果表明,玉米素有助于茎段和试管薯转化再生芽的分化,乙酰丁香酮可以提高茎段的转化率,但对试管薯的转化作用效果不明显。卡那霉素生根筛选和PCR鉴定结果显示,在分化培养基上再生的转化植株假阳性率较高,在遗传转化工作中要加大转基因植株的数量,才有可能获得所期望的转基因植株群体。

AN EFFICIENT TRANSFORMATION SYSTEM OF POTATO MEDIATD BY AGROBACTERIUM TUMEFACIENS

An efficient transformation system has been established for Agrobacterium-mediated transformation of potato cultivar“Favorita”with transformation efficiency of 25.6% and 36.8% for internode and microtuber, respectively. The results showed that zeatin is beneficial to shoot regeneration of transformed internode and microtuber. Acetosyringone could increase transformation efficiency of internode while has not an apparent effect on microtuber. Rooting selection on kanamycin and PCR identification demonstrated that the false positive efficiency of transformation is higher among the putative transgenic plants from the differentiation media,and suggested that the desired transgenic plants could be obtained from a large number of transgenic plants in potato transformation.