| 间接ELISA检测禽多杀性巴氏杆菌抗体中包被抗原的选择 |
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| 引用本文： | 龚建森,施祖灏,单艳菊,陆俊贤,葛庆联,刘学贤.间接ELISA检测禽多杀性巴氏杆菌抗体中包被抗原的选择[J].吉林畜牧兽医,2008,29(1):10-12. |
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| 作者姓名： | 龚建森 施祖灏 单艳菊 陆俊贤 葛庆联 刘学贤 |
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| 作者单位： | 江苏省家禽科学研究所,江苏扬州,235003 |
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| 基金项目： | 江苏省“十一五”农业科技攻关项目(BE2006363) |
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| 摘 要： | 间接ELISA已在抗体检测中广泛应用，而抗原包被作为间接ELISA中必不可少的一步可直接影响试验的准确性。本试验分别使用超声波裂解抗原、脂多糖蛋白抗原和全茵抗原作为包被抗原，建立相应的检测方法并进行重复性试验。试验证明全菌抗原建立的禽多杀性巴氏杆菌ELISA检测方法在敏感度、特异性、稳定性方面均优于其他两种抗原。
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| 关 键 词： | 禽多杀性巴氏杆菌 包被抗原 ELISA |
The Selection of Coating Antigen in Indirect ELISA for Detection of Serum Antibodies Against Pasteurella multocida |
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| GONG Jian-sen,SHI Zu-hao,SHAN Yan-ju,LU Jun-xian,GE Qing-lian,LIU Xue-xian.The Selection of Coating Antigen in Indirect ELISA for Detection of Serum Antibodies Against Pasteurella multocida[J].Jilin Animal Science and Veterinary Medicine,2008,29(1):10-12. |
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| Authors: | GONG Jian-sen SHI Zu-hao SHAN Yan-ju LU Jun-xian GE Qing-lian LIU Xue-xian |
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| Institution: | (The Institute of Poultry Science of Jiangsu Province, Yangzhou Jiangsu 235003) |
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| Abstract: | The indirect ELISA is widely used for detection of antibodies and coating antigen is very important to the accu-racy of ELISA experiment. The experiment applied sonicate antigen, lipopolysaccharides protein antigen and whole bac-terial antigen as the coating antigen, use indirect ELISA for detection of antibody against fowl cholera, and make the repli-cate test by the different antigens. The experiment proved the sensitivity, speaciality and repeativity of the whole bacterial antigen was better than the others antigen. |
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| Keywords: | pasteurella multocida coating antigen ELISA |
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