Cluster analysis of 36Globodera pallida field populations using two sets of molecular markers |
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Authors: | Rolf T. Folkertsma Koen E. de Groot Paul H. G. van Koert Marga P. E. van Gent-Pelzer Jeroen N. A. M. Rouppe van der Voort Arjen Schots Jaap Bakker Fred J. Gommers Johannes Helder |
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Affiliation: | (1) Dept. of Nematology, Wageningen Agricultural University, P.O. Box 8123, 6700 ES Wageningen, The Netherlands;(2) Present address: Res Inst. for Plant Protection, IPO-DLO, P.O. Box 9060, 6799 GW Wageningen, The Netherlands |
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Abstract: | Thirty-six populations of the potato cyst nematodeGlobodera pallida, all collected in the Netherlands, were analysed twice: by two-dimensional gel electrophoresis of proteins (2-DGE) and by random amplified polymorphic DNA fingerprinting (RAPD). Two-DGE revealed frequencies of 21 alleles at eight putative loci in each population. The same populations were subjected to RAPD analysis. This qualitative technique revealed 38 polymorphic DNA fragments. Both datasets were independently processed to determine the intraspecific variation. UPGMA analysis resulted in a 2-DGE- and a RAPD-based dendrogram with cophenetic correlation coefficients of 0.755 and 0.838 respectively. The correlation between the genetic similarity values for the populations was 0.572. Comparison between the 2-DGE- and the RAPD-based dendrogram revealed that only thirteen of the 36 populations analysed were clustered identically. It is concluded that the gene pool similarity concept is only in some instances applicable to Dutch populations ofG. pallida. For populations that could not be differentiated unequivocally on the basis of molecular markers, markers closely linked to avirulence genes should be identified. Approaches that will lead to the identification of such markers are discussed. |
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Keywords: | genetic variation potato cyst nematode random amplified polymorphic DNA two-dimensional gel electrophoresis of proteins |
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