| 白菜型油菜PABP5基因启动子的克隆及表达特性分析 |
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| 引用本文： | 石磊,董彩华,白泽涛,郭学兰,柴国华,黄军艳,刘胜毅. 白菜型油菜PABP5基因启动子的克隆及表达特性分析[J]. 分子植物育种, 2009, 7(2) |
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| 作者姓名： | 石磊 董彩华 白泽涛 郭学兰 柴国华 黄军艳 刘胜毅 |
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| 作者单位： | 中国农业科学院油料作物研究所,农业部油料作物生物学重点开放实验室,武汉,430062 |
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| 摘 要： | 本研究中利用基因组步移法从白菜型油菜中克隆到Poly(A)结合蛋白基因PABP5起始密码子上游长588 bp的一段序列,经软件分析预测表明,该序列具有典型的启动子结构,并且具有多个与花药特异性表达相关的顺式作用元件,如TATA box,CAAT box,AGAAA motif,AGGTCA motif,AAACCCTAA motif,GTGA motif等.为了研究该片段的表达特性,本文将克隆到的序列置换pPBI121中的CaMV35S启动子,驱动其下游的GUS基因,构建了植物表达载体,转化拟南芥,获得了转基因植株.组织化学染色表明,在PABP5启动子Ppabp5的驱动下,报告基因GUS在拟南芥的根尖、第一片真叶叶原基以及花药中特异表达.本研究为PABP5基因的进一步功能研究奠定了基础.
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| 关 键 词： | 白菜型油菜 启动子 拟南芥 花药特异性表达 |
Isolation of a Brassica rapa Promoter of Gene PABP5 and its Function Analysis in Transgenic Arabidopsis thaliana |
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| Shi Lei,Dong Caihua,Bai Zetao,Guo Xuelan,Chai Guohua,Huang Junyan,Liu Shengyi. Isolation of a Brassica rapa Promoter of Gene PABP5 and its Function Analysis in Transgenic Arabidopsis thaliana[J]. Molecular Plant Breeding, 2009, 7(2) |
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| Authors: | Shi Lei Dong Caihua Bai Zetao Guo Xuelan Chai Guohua Huang Junyan Liu Shengyi |
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| Affiliation: | Shi Lei Dong Caihua Bai Zetao Guo Xuelan Chai Guohua Huang Junyan Liu Shengyi Key Laboratory for Biological Sciences of Oil Crops,the Ministry of Agriculture,Oil Crops Research Institute,Chinese Academy of Agricultural Sci- ences,Wuhan,430062 |
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| Abstract: | 5'flanking sequence of Brassica rapa Poly(A)binding protein gene PABP5 was cloned by using PCR-based genomic walking method. Results of sequence analysis showed that the cloned sequence contained several pu- tative cis-elements for anther-specific activity, such as TATA box, CAAT box, AGAAA motif, AGGTCA mo- tif, AAACCCTAA motif, GTGA motif, GCGGGAAA motif and GGGCC motif, as well as several motif for ABA responding. A plant expression vector containing a Ppabp5-GUS fusion construct was constructed and tran... |
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| Keywords: | PABP5 |
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