用于虫生真菌遗传转化的苯菌灵抗性菌株筛选

发展虫生真菌的抗药性是协调生防与化防,稳定真菌杀虫剂应用效果的一个重要策略。本研究从代表性地区采集植物病原样本,分离得到35株菌株,鉴定为3属5种,即灰葡萄孢霉Botrytis cinerea、扩展青霉Penicillium expansum、指状青霉Penicillium digitatum、长柄链格孢菌Alternaria longipes和茄链格孢菌Alternaria solani。应用菌落直径法测定其对苯菌灵的抗性,结果表明：供试的11株灰葡萄孢霉菌株中,最高EC50为936.2670μg·mL^-1;12株青霉属菌株中,最高EC50为0.0384μg·mL^-1;12株链格孢菌株中,最高EC50为703.5557μg·mL^-1。其中灰霉和链格孢属菌株中有4株EC50大于600μg·mL^-1的高抗菌株,即灰葡萄孢霉的菌株SD2和SD1以及茄链格孢的菌株CC2-2和LF1-2,这4株菌株可作为遗传转化抗性基因克隆的基础材料。

Screening of Benomyl Resistant Strains for Genetic Transformation of Entomogenous Fungi

One strategy to stabilize the performance of fungal pesticides in case of chemical pesticides,is to develop the resistance of the entomopathogenic fungi to fungicides.Thirty-five phytopathogenic fungal isolates were collected from different areas of China.The isolates were identified as 5 species of 3 genus,namely Botrytis cinerea,Penicillium expansum,Penicillium digitatum,Alternaria longipes and Alternaria solani,respectively.The benomyl resistance of the isolates was determined by the colony radial growth inhibition method.The maximum EC50 values of Botrytis,Penicillium and Alternria were 936.2670,0.0384 and 703.5557 μg·mL 1,respectively.Two isolates（SD1 and SD2） of B.cinerea and two isolates（CC2-2 and LF1-2） of A.solani were screened,with EC50 values of over 600 μg·mL 1.These isolates were considered as the potential materials to clone the resistant genes for the transformation of entomopahtogenic fungi.