A Simple Method for Preparation of Rice Genomic DNA |
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| Authors: | SUN Chuan # HE Ying-hong CHEN Gang RAO Yu-chun ZHANG Guang-heng GAO Zhen-yu LIU Jian JU Pei-na HU Jiang GUO Long-biao QIAN Qian ZENG Da-li |
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| Affiliation: | SUN Chuan 1,2,#,HE Ying-hong 1,CHEN Gang 1,RAO Yu-chun 1,ZHANG Guang-heng 1,GAO Zhen-yu 1,LIU Jian 1,JU Pei-na 1,HU Jiang 1,GUO Long-biao 1,QIAN Qian 1,ZENG Da-li 1(1 State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China,2 Agricultural College,Yangzhou University,Yangzhou 225009,# These authors contributed equally to this paper) |
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| Abstract: | The extraction of DNA is often the most time consuming and laborious step in high-throughput molecular genetic analysis and marker assisted selection (MAS) programs. A simple method for preparation of rice genomic DNA was developed. A small amount (1-50 mg) of leaf tissue of rice seedling, 500 μL of extraction buffer, and one steel bead were put into a 2-mL microcentrifuge tube. After vigorously mashing for 2 min, 5 μL of supernatant was directly applied to PCR amplification. Otherwise, the supernatant was precipitated with two times volume of ethanol to obtain high quality genomic DNA. This method is simple, rapid, low cost, and reliable for PCR analysis. One person can manipulate as many as 96 samples for PCR in 10 min. It is especially suitable for genotyping of large number of samples. |
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| Keywords: | DNA extraction high-throughput PCR marker assisted selection gene mapping rice |
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