山羊催乳素受体基因部分片段的克隆与序列分析

采用PCR技术扩增出济宁青山羊催乳素受体基因长892 bp的片段，该片段含有97 bp的部分外显子8序列（外显子8全长为100 bp）、683 bp的内含子8、70 bp的外显子9及42 bp的部分内含子9。将该片段克隆到pGEM-T Easy质粒中，重组质粒用PCR进行阳性克隆鉴定，然后测定核苷酸序列，并推导其氨基酸序列。该序列与绵羊、母牛、人、大鼠、小鼠的催乳素受体基因mRNA的对应序列的核苷酸同源性分别为99.4 %、97.01%、89.22%、89.22%、88.02%，氨基酸同源性分别为100%、94.55%、81.88%、81.82%、83.64%。

Cloning and Sequence Analysis of Partial Fragment of Prolactin Receptor Gene in Goats

The 892 bp fragment(97 bp of part of exon 8(the full length of exon 8 was 100 bp),683 bp of intron 8,70 bp of exon 9 and 42 bp of part of intron 9)of prolactin receptor(PRLR) gene was amplified successfully in Jining Grey goats by PCR and cloned into pGEM-T Easy vector.The positive clones were further identified by PCR analysis.The nucleotide sequence was detected and the peptide sequence of this fragment was deduced.This sequence shared 99.4 %,97.01%,89.22%,89.22%,88.02% nucleotide homology with the published mRNA of PRLR gene of sheep,cow,human,rat and mouse separately,and the amino acid homology was 100%,94.55%,81.88%,81.82%,83.64% separately.