愈伤组织再生马铃薯脱毒苗生产体系的建立

以马铃薯茎尖、茎段、全叶、半叶为外植体,进行愈伤组织的诱导、继代、根芽分化和植株再生的研究,建立马铃薯脱毒再生体系。结果表明:(1)马铃薯茎尖、茎段、半叶、全叶在MS+6-BA1.5 mg·L-1+NAA1 mg·L-1+3%蔗糖+0.6%琼脂培养基中,愈伤组织诱导率分别达到97.3%、75.6%、96.8%、97.4%;(2)在MS+6-BA1.5mg·L-(1或ZT2.0 mg·L-1)+NAA0.5 mg·L-1+GA3 5 mg·L-1+3%蔗糖+0.6%琼脂培养基中不同外植体芽诱导率在15.4%～67.7%,根的分化率21.3%～88.2%。

Establishment of Production System for Regenerated Virus-free Potato Plantlets through Callus Tissue

Callus industion,subculture,root and bud differentfiation and plant regeneration was carried out with stem tip,stem section and leaf blade as explants in order to establish production system for regeneration of virus-free potato plantlets from callus.In the medium MS+6-BA 1.5 mg·L-1+NAA 1 mg·L-1+3% sucrose+0.6% agar,induction rate of callus for potato stem tip,stem section,whole leaf and half leaf was 97.3%,75.6%,96.8% and 97.4%,respectively.In the medium MS+6-BA1.5 mg·L-1(or ZT2.0mg·L-1)+NAA 0.5 mg·L-1+GA3 5 mg·L-1+3% sucrose +0.6% agar,bud induction rate of various explants was 15.4%~67.7%,and differentiation rate of root was 21.3%~88.2%.