部分箬竹属植物的叶绿体DNA分析

目的]研究箬竹属植物和赤族属植物的亲缘关系。方法]以箬竹属13个竹种及其近缘的赤竹属3个竹种为材料,采用PCR方法扩增叶绿体trnL-trnF间隔区基因片段,并对其进行序列分析,构建系统树。结果]利用已发表的trnL-trnF序列通用引物扩增出长度为1008～1103bp的trnL-trnF片段,比较长度为940bp。cpDNA序列聚类将箬竹属与赤竹属竹种混合聚在一起,同源性为99%以上,可分为5组。其中,髯毛箬竹、广东箬竹、小叶箬竹、华箬竹、毛鞘箬竹、矮箬竹、胜利箬竹、箬竹、箬叶竹、粽巴箬竹、翠竹和菲白竹12个竹种聚为一组;泡箬竹、天目箬竹、阔叶箬竹和美丽箬竹4个竹种各自成一组。结论]竹种间的同源性极高,表现为较慢的进化速率,提供的信息位点很少,不能很好地解决箬竹属种间的系统学问题。

Genetic Diversity of Indocalamus Determined by Chloroplast DNA Sequence

Objective]The aim was to research affinity between Indocalamus and Sasa.Method]Using 13 samples of Indocalamus and 3 samples of Sasa as materials,the intergenic regions of trnL-trnF gene in chloroplast were amplified by PCR,and sequence analysis and phylogenetic trees construction were carried out.Result]Usigng the universal primer,the intergenic regions of trnL-trnF were amplified,the lengths of the segments varied from 1 008 bp to 1 103 bp,of which 940 bp was compared. The dendrogram of trnL-trnF sequences showed that Indocalamus and Sasa were clustered together and they were homologous by 99%. All the samples were divided into five groups,the first group included 12 sapmples such as Indocalamus pedalis,I. pumilus,I. victorialis,I. longiauritus,I. tessellatus,Sasa sinica,Sasa pygmaea,I. barbatus,I. guangdongensis,I. herklotsii,I. Hirtivaginatus and S. fortunei. I. decorus,I. lacunosus,I. Latifolius and I. Migoi were respectively divided into four groups. Conclusion]The high homology of all samples showed the low evolution speed and little information sites which suggested that the phylogeny of Indocalamus could not be well resolved by the intergenic region of trnL-trnF.