| 含B染色体的黑麦基因组DNA甲基化变异研究 |
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| 引用本文： | 孙晓明,魏丽华,李象松,龙鸿. 含B染色体的黑麦基因组DNA甲基化变异研究[J]. 安徽农业科学, 2007, 35(22): 6733-6736 |
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| 作者姓名： | 孙晓明 魏丽华 李象松 龙鸿 |
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| 作者单位： | 华中农业,大学生命科学技术学院,湖北武汉,430070;华中农业,大学生命科学技术学院,湖北武汉,430070;华中农业,大学生命科学技术学院,湖北武汉,430070;华中农业,大学生命科学技术学院,湖北武汉,430070 |
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| 摘 要： | 运用RAPD法检测黑麦JNK居群中含有和不含B染色体的基因组DNA的序列差异,并从100个10碱基随机引物中筛选出5个,采用CRED-RA法,检测含有和不含B染色体基因组DNA的甲基化变异。结果表明,含有和不含B染色体的黑麦基因组中均可以发生CmCGG甲基化修饰,但与不含B染色体的基因组相比,含有B染色体的黑麦基因组中发生了更广泛的CmCGG甲基化变异,这可能是B染色体产生表型抑制或基因沉默的一个重要原因。
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| 关 键 词： | B染色体 DNA甲基化 RAPD 黑麦 |
| 文章编号： | 0517-6611（2007）22-06733-04 |
| 修稿时间： | 2007-04-03 |
Variation of DNA Methylation in B Chromosomes of Rye |
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| SUN xiao-mingetal. Variation of DNA Methylation in B Chromosomes of Rye[J]. Journal of Anhui Agricultural Sciences, 2007, 35(22): 6733-6736 |
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| Authors: | SUN xiao-mingetal |
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| Abstract: | DNA sequence differences in rye genomes(JNK population) with and without B chromosomes were analyzed by random amplified polymorphic DNA(RAPD).5 out of 100 arbitrary 10-mer primers were screened out,following coupled restriction enzyme digestion and random amplification(CRED-RA) of genomic DNA to detect cytosine methylation changes in genomes with and without B chromosomes.The results showed that DNA methylation(CmCGG) was observed in both genomes with and without B chromosomes.Moreover,genomes with B chromosomes caused extensive DNA methylation(CmCGG) comparing with those without B chromosomes.These epigenetic changes might play a key role in the phenotypic suppression or gene silencing caused by B chromosome. |
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| Keywords: | B chromosome DNA methylation RAPD Secale cereale L. |
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