猪睾丸间质细胞的分离及培养

为丰富体细胞核移植的供体细胞种类,本研究对猪睾丸间质细胞的分离和培养方法进行了系统研究。运用酶消化法和钢网过滤筛选法获得的猪睾丸细胞,呈现圆形、细胞体积较大,培养4~6 h后开始贴壁;培养48 h后,贴壁增殖;培养3~5 d后可形成单层细胞。这一研究结果为研究和建立睾丸间质细胞体外培养体系提供技术方法和试验依据。

Isolation and in vitro culture of porcine leydig cell

In order to enrich the donor variety of the somatic cell nuclear transfer,methods for isolation and culture of porcine testis leydig cell were investigated in this study.Porcine testis leydig cells isolated by enzymatic digestion and steel net filtration were round and large.It began to attach clumps after 4～6 h of culture,proliferated to form monolayers at 48 h of culture,and confluented on 3～5 d of culture.These results will provide a basis for isolation and in vitro culture of porcine testis eydig cells.