Detection and antigenic characterization of salmonid alphavirus isolates from sera obtained from farmed Atlantic salmon, Salmo salar L., and farmed rainbow trout, Oncorhynchus mykiss (Walbaum) |
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| Authors: | Jewhurst V A Todd D Rowley H M Walker I W Weston J H McLoughlin M F Graham D A |
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| Institution: | Department of Veterinary Science, The Queen's University of Belfast, Veterinary Sciences Division, Stormont, Belfast, UK; Veterinary Sciences Division, Department of Agriculture and Rural Development, Stormont, Belfast, UK; Aquatic Veterinary Services, Cherryvalley Park, Belfast, UK |
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| Abstract: | A simple method of detecting the presence of the salmonid alphaviruses (SAVs), salmon pancreas disease virus (SPDV) and sleeping disease virus (SDV), from serum samples is described. Using a 96-well tissue-culture plate format, test sera are diluted in medium and added to chinook salmon embryo (CHSE-214) cells. After incubation for 3 days at 15 degrees C, plates are fixed and stained using a monoclonal antibody (mAb)-based immunoperoxidase (IPX) detection system, and virus-infected cells are observed microscopically by white light. Application of this screening test, which is now used routinely in our laboratory in conjunction with an IPX-based virus neutralization (IPX-VN) test for detecting antibodies to SAVs, has resulted in the recovery of 12 additional isolates from salmon sera and four additional isolates from trout sera. A low level of antigenic variation was detected when these SAV isolates were investigated by indirect immunofluorescence using a panel of mAbs raised to reference SPDV and SDV isolates. |
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| Keywords: | alphavirus salmon pancreas disease salmon pancreas disease virus sleeping disease sleeping disease virus viraemia |
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