| 铁皮石斛原球茎增殖培养基配方的优化 |
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| 引用本文： | 戴传云,刘腾飞,管天冰,刘万宏.铁皮石斛原球茎增殖培养基配方的优化[J].安徽农业科学,2011,39(10):5788-5789. |
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| 作者姓名： | 戴传云 刘腾飞 管天冰 刘万宏 |
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| 作者单位： | 重庆科技学院生物系,重庆,401331;重庆科技学院生物系,重庆,401331;重庆科技学院生物系,重庆,401331;重庆科技学院生物系,重庆,401331 |
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| 摘 要： | 目的]获得铁皮石斛（Dendrobium candidum Wall.ex Lindl.）原球茎组织培养的最佳培养基配方。方法]用正交试验筛选出6-BA、NAA、KT最佳的浓度配比,并考察各因素对铁皮石斛原球茎增殖率的影响。结果]铁皮石斛原球茎增殖的最佳培养基配方为1/2MS＋2.0 mg/L 6-BA＋0.5 mg/L NAA＋1.0 mg/L KT。结论]该研究为工业化生产铁皮石斛原球茎及其有效成分,推进铁皮石斛工厂化育苗奠定了基础。
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| 关 键 词： | 铁皮石斛 原球茎 增殖 正交试验 |
Optimization of Medium Formula for Protocorm Proliferation of Dendrobium candidum Wall.ex Lindl |
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| Institution: | DAI Chuan-yun et al(Department of Biology,Chongqing University of Science and Technology,Chongqing 401331) |
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| Abstract: | Objective] To obtain the optimum medium formula for protocorm proliferation of Dendrobium candidum Wall.ex Lindl..Method] The optimal concentration formula of 6-BA,NAA and KT was screened by orthogonal test,and effect of each factor on proliferation rate of protocorm from Dendrobium candidum Wall.ex Lindl.was investigated.Result] The optimum medium formula was:1/2MS+2.0 mg/L 6-BA+0.5 mg/L NAA+1.0 mg/L KT.Conclusion] This study provided basis for industrialized production of protocorm proliferation of Dendrobium candidum Wall.ex Lindl.and its active compositions,and for advancing the industrialized breeding of Dendrobium candidum Wall.ex Lindl.. |
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| Keywords: | Dendrobium candidum Wall ex Lindl Protocorm Proliferation Orthogonal test |
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