银杏叶黄酮提取工艺的优化

目的]优化银杏叶总黄酮的提取工艺,为银杏黄酮的精制提供前提条件。方法]采用紫外分光光度法测定银杏叶中总黄酮的含量;用单因素试验考察乙醇浓度、回流温度、提取时间对银杏黄酮收率的影响,优化提取条件。结果]银杏叶中总黄酮含量为1.502 6mg/ml;最佳提取条件：70%乙醇为提取剂,回流温度为80℃,提取时间为3.0 h。结论]该研究得到银杏黄酮最佳的提取工艺,为银杏叶黄酮的精制提供了前提条件。

Optimization of Extraction Process of Flavonoids from Ginkgo biloba L. Leaf

Objective] To optimize the extraction process of flavonoids from Ginkgo biloba L.leaf,so as to provide basis for the refining of Ginkgo biloba L.leaf flavonoids.Method] Content of flavonoids was determined by UV spetrophotometry.By employing single factor test,effect of ethanol concentration,reflux temperature and extraction duration on yield of flavonoids from Ginkgo biloba L.leaf,optimizing the extraction conditions.Result] The optimal extraction conditions were determined to be: extracting solvent of 70% ethanol,extraction temperature of 80 ℃,extraction duration of 3.0 h.Under the optimum extraction process,content of flavonoids in Ginkgo biloba L.leaf reached 1.520 6 mg/ml.Conclusion] The optimal extraction conditions were obtained in this study,which provided basis for refining of Ginkgo biloba L.leaf flavonoids.