| 枯草芽孢杆菌B_2种子液发酵条件的研究 |
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| 引用本文： | 闫沛迎,陈秀蓉,薛莉,杨成德.枯草芽孢杆菌B_2种子液发酵条件的研究[J].甘肃农业大学学报,2008,43(2):110-113. |
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| 作者姓名： | 闫沛迎 陈秀蓉 薛莉 杨成德 |
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| 作者单位： | 甘肃农业大学草业学院,甘肃,兰州,730070 |
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| 基金项目： | 甘肃省生物技术专项基金
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甘肃省555创新人才工程 |
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| 摘 要： | 采用单因子、双因子及正交试验,对枯草芽孢杆菌B2的培养液组成(碳源、氮源、酵母膏、pH值)及培养条件(装液量与转速)进行了研究,结果表明:以葡萄糖1%,牛肉胨0.8%,酵母膏0.5%及pH 8制成的培养液,装液量100 mL/250 mL锥形瓶,转速140 r/min,培养B236 h为最佳培养条件,在此条件下,活菌数可达2.97×1010cfu/mL,分别是D培养液和NB培养液的2.05倍和52.36倍.
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| 关 键 词： | 枯草芽孢杆菌B2 培养基组成 培养条件 优化 |
| 文章编号： | 1003-4315(2008)02-0110-04 |
| 修稿时间： | 2007年4月16日 |
Fermentation conditions of Bacillius subtilis B2 |
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| YAN Pei-ying,CHEN Xiu-rong,XUE Li,YANG Cheng-de.Fermentation conditions of Bacillius subtilis B2[J].Journal of Gansu Agricultural University,2008,43(2):110-113. |
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| Authors: | YAN Pei-ying CHEN Xiu-rong XUE Li YANG Cheng-de |
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| Abstract: | The medium components and culture conditions were investigated through single-factor,double factors and orthogonal tests.The experiments showed the optimum fermentation medium was composed of glucose 10 g,beef peptone 8 g,yeast extract paste 5 g,pH 8,the culture condition was 100 mL inventory/250 mL flask matching to the rotate speed of 140 r/min,the optimum fermentation time 36 h.The active bacterium quantity produced under the condition was about two-fold than that in D medium and about fifty-fold than that in NB medium. |
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| Keywords: | Bacillius subtilis B2 medium components culture conditions optimization |
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