50例稽留流产绒毛染色体分析和实验方法的改进

为改进绒毛组织细胞培养方法和染色体制备方法,探讨染色体改变与稽留流产的关系,对50例稽留流产绒毛组织进行培养。每例分为实验组和对照组。两组标本均先用胰酶消化后再培养5~10 d,对照组按常规法收获细胞,实验组将细胞传代后采用改良方法进行收获。两组细胞染色体制片完成后进行G显带分析,分别计算并比较分裂指数。结果表明,实验组细胞收获方法步骤简便易于操作,分裂指数较对照组高,染色体形态和分散程度均较对照组好。50例绒毛组织染色体分析发现常染色体异常14例,性染色体异常8例,染色体结构异常3例,三倍体1例,正常核型24例。

Chromosome Analysis of Chorionic Villi in 50 Cases of Missed Abortions and Improvement of an Experiment Method

Chorionic villi tissues from 50 cases of missed abortions were sampled and cultured to improve methods for tissue culture and chromosome preparation and to analyze the relationship between the chromosome change and the missed abortion.All the samples are divided into both control and experiment groups each.They were cultured for 5 ～ 10 days after trypsin digestion.The cells from the chorionic villi tissues in the control group were harvested by using the conventional method,while the cells in the experiment group were then subcultured and harvested by using a modified method.The cells from these two groups were analysed by G-banding after their chromosomes were prepared,and their mitotic indexes were calculated and compared.The results showed that the harvesting method in the experiment group is simple and easy to operate and produces higher mitotic indexes and better chromosome morphology and dispersion than the conventional method in the control group.Analysis of the chorionic villi from these 50 cases of missed abortions showed that 14 cases are of autosomal anomalies,8 cases of sex chromosome abnormalities,3 cases of chromosomal structural abnormality,1 case of triploid,and 24 cases of normal karyotypes.