| 海南珠母贝属Pinctada6个种的分类鉴定 |
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| 引用本文： | 温海洋,;石耀华,;顾志峰,;战欣,;王爱民.海南珠母贝属Pinctada6个种的分类鉴定[J].热带生物学报,2014(1):1-7. |
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| 作者姓名： | 温海洋 ;石耀华 ;顾志峰 ;战欣 ;王爱民 |
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| 作者单位： | [1]海南大学热带生物资源教育部重点实验室,海南海口570228; [2]海南大学海洋学院/海南省热带水生生物技术重点实验室,海南海口570228 |
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| 基金项目： | 国家重点基础研究发展计划（973计划）（2010CB126405）;海南省青年基金项目（313033）;海南大学科研启动基金资助项目（kyqd1216）;海南大学青年基金资助项目（qnjj1227） |
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| 摘 要： | 通过测试海南省珠母贝属Pinctada 6个种的45个个体的16S和H3基因序列,并与GenBank数据库中相同物种进行比对,构建系统发育树对其进行分类,同时根据简单、明确的贝壳形态学特征予以最终确认。此分类法不仅可以较准确、快速地进行珠母贝属的分类鉴定,而且分类结果不易受被测试贝类生活环境的影响,不易受分类鉴定人员水平的限制,有利于发现杂交种和隐存种。
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| 关 键 词： | 珠母贝属 S H 形态特征 分类鉴定 |
Classification and Identification of Six Pinctada Species in Hainan Province |
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| Institution: | WEN Haiyang, SHI Yaohua, GU Zhifeng, ZHAN Xin, WANG Aimin ( 1. Ministry of Education Key Laboratory of Tropical Bio-resourees, Hainan University, Haikou 570228, China;2. Ocean College/Hainan Key Laboratory of Tropical Hydrobiologieal Technology, Hainan University, Haikou 570228, China) |
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| Abstract: | A total of 45 individuals from six species of pearl oysters （Pinctada spp. ） were sequenced based on gene 16S and geneH3. NJ trees were constructed by the 45 tested oysters and the individuals from the GenBank database using 16S andH3 sequences. Most of the 45 tested oysters were clustered with the identical species from the GenBank database. With the help of simple and clear shell features, 6 species of Pinctada collected from Hainan Province were identified. This classification method could not only help identify quickly and precisely species of Pinctada, and avoid influences of environmental and human factors, but also could facilitate crossbreeding and discovery of cryptic species of Pinctada. |
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| Keywords: | Pinctada 16S H3 shell feature classification and identification |
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