| 牛轮状病毒的分离及其RT-PCR鉴定 |
| |
| 引用本文： | 姜向阳,邓小敏,王晶钰,王学艳,罗艳.牛轮状病毒的分离及其RT-PCR鉴定[J].西北农业学报,2007,16(4):191-194. |
| |
| 作者姓名： | 姜向阳 邓小敏 王晶钰 王学艳 罗艳 |
| |
| 作者单位： | 西北农林科技大学动物科技学院,陕西杨凌,712100 |
| |
| 摘 要： | 采用MAl04细胞从疑似轮状病毒腹泻犊牛粪样中进行轮状病毒分离，盲传4代后接种细胞出现明显细胞病变（CPE），对分离病毒进行核酸聚丙烯酰胺凝胶电泳，电泳条带为4：2：3：2型，具备典型A群轮状病毒特征。依据NCBI等登录的牛轮状病毒的VPl基因序列设计一对引物，对其VPl基因进行克隆及序列分析，结果分离毒与国外牛轮状病毒分离株RF的核苷酸同源性为98．9％，与UK的核苷酸同源性为91．5％，证明分离毒株是一株牛轮状病毒。
|
| 关 键 词： | 轮状病毒 细胞培养 VP1基因 MA104细胞 |
| 文章编号： | 1004-1389（2007）04-0191-04 |
| 收稿时间： | 2006-11-17 |
| 修稿时间： | 2006-11-172007-03-10 |
Isolation of Bovine Rotavirus and Identification by Using RT-PCR |
| |
| JIANG Xiang-yang,DENG Xiao-min,WANG Jing-yu,WANG Xue-yan and LUO Yan.Isolation of Bovine Rotavirus and Identification by Using RT-PCR[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(4):191-194. |
| |
| Authors: | JIANG Xiang-yang DENG Xiao-min WANG Jing-yu WANG Xue-yan and LUO Yan |
| |
| Institution: | College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China;College of Animal Science and Technology, Northwest A & F University, Yangling Shaanxi 712100, China |
| |
| Abstract: | One strain of bovine rotavirus was isolated from excrement of calf by using MA104 cell,and produced obvious cytopathic effects after fostering 4 times.RNA of isolated virus was analysed by PAGE and was type of 4:2:3:2,which belonged to rotavirus A type.A pair of primers were designed to clone VP1 gene according to sequences of bovine rotavirus in NCBI.The results showed that nucleotides sequence of VP1 shared 98.9% with RF isolated strain,91.5% with UK. |
| |
| Keywords: | Rotavirus Cell culture VP1 gene MA104 cell |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《西北农业学报》浏览原始摘要信息 |
| 点击此处可从《西北农业学报》下载免费的PDF全文 |
|
