绵羊MXD3基因的生物信息学分析

MAX二聚化蛋白3(MAX dimerization protein 3, MXD3)在肿瘤的增殖、凋亡、侵袭和转移过程中起重要作用，广泛存在于多种动物体内。为探究MXD3基因在绵羊体内的功能，运用生物信息学数据库及其软件，分析了该基因及其编码的产物。结果发现，绵羊MXD3基因总共编码了206个氨基酸残基，所编码的蛋白质分子式为C1000H1650N332O315S6，分子质量为23 556.50 KDa，理论等电点pI为9.32，半衰期为30 h，不稳定指数为88.30。通过基本理化性质分析可知，绵羊MXD3主要在细胞核发挥作用，不属于分泌蛋白，不存在信号肽序列，不存在跨膜结构，是亲水性蛋白。该蛋白的二级结构主要是以无规卷曲、α螺旋组成，而三级结构预测结果与二级结构相符。

Bioinformatics Analysis of Ovine MXD3 Gene

MAX dimerized protein 3(MXD3) gene plays an important role in tumor proliferation, apoptosis, invasion and metastasis, which is widely present in a variety of animals. In order to explore the function of MXD3 gene in sheep, bioinformatics analysis of MXD3 gene and its encoded products was conducted by using bioinformatics databases and software. The results showed that MXD3 gene encoded 206 amino acid residues, the protein molecular formula was C1000H1650N332O315S6, the molecular weight was 23 556.50 KDa, isoelectric point pI was 9.32, the half-life was 30 h, and the instability index was 88.30. Sheep MXD3 protein subcells were mainly located in nucleus, which was not a secreted protein and therefore no signal peptide sequence was detected. No transmembrane structure was found and was regarded as a hydrophilic protein. The secondary structure of the protein was composed of random coil and α helix, and the predicted results of tertiary structure were consistent with the secondary structure.