复合寡糖施用对促进黄瓜生长与改善细菌群落结构的影响

植物刺激素寡糖具有提高作物抗逆作用、促进作物生长的功效。在黄瓜生长早期及生长期施用复合寡糖制剂，通过分析复合寡糖对植株光合作用特性、产量和黄瓜3个生长阶段植株叶片、根系及根围土微生物群落，阐明复合寡糖施用对黄瓜的增产增效作用。采用大棚小区试验，设置清水对照、化学常规和复合寡糖3个处理，于盛花期、盛果期和拉秧期分别采集黄瓜叶片、根系和根围土样本，测定各个采样时期黄瓜产量、叶绿素含量和抗氧化酶活性，进行高通量测序检测样本细菌群落结构和组成。复合寡糖的施用与清水对照相比，提高了单株黄瓜产量，使得每公顷的产量增加了4.96%；显著提高了黄瓜叶片叶绿素的含量，复合寡糖处理对比其他处理能够显著提升叶片叶绿素含量的相对值（SPAD）6.14%～11.46%；同时提高了叶片的超氧化物歧化酶活性39.9%～81.5%，提高了过氧化氢酶活性8.4%～40.4%，提高了过氧化物酶活性1.19%～18.5%。高通量测序分析结果表明，复合寡糖的施用提高了叶片微生物群落中鞘氨醇单胞菌属（Sphingomonas sp.）和甲基杆菌属（Methylobacterium sp.）的丰度，它们均具有促进植物生长和协助植...

Effect on bacterial community of complex oligosaccharide applied in cucumber growth system

Oligosaccharides，a kind of plant stimulatinghormone，had the effects of improving crop stress resistance and promoting cropgrowth.To elucidate the effects of complex oligosaccharides application on increasing yield and efficiency of cucumberplants，the effects of complex oligosaccharides on plant photosynthesischaracteristics，yield andthe microbial communities in the leaves，roots，and soil around the roots of cucumber at three growth stages were analyzed after complex oligosaccharides preparations applied in the early and growth stages ofcucumber.Using a greenhouseplot experiment，three treatment groups were setup:clear watercontrol，chemical conventional treatment and complex oligosaccharidetreatment.Cucumberleaves，roots，and soil samples were collected during theflowering，fruiting and seedlingstages，respectively.Cucumberyield，chlorophyll content and antioxidant enzyme activity were measured at eachsamplingperiod，and high -throughput sequencing was performed to detect the bacterial community structure and composition of thesamples.Compared with clear watercontrol，the application of complex oligosaccharides increased theyield of cucumber per plant by4.96%.The content of chlorophyll in cucumber leaves wassignificantlyincreased，and the relative value of chlorophyllcontent(SPAD)in the complex digosaccharide treatment was significantly increased by 6.14%-11.46% compared toothertreatments.At the sametime，the activity of superoxide dismutase in the leaves was increasedby39.9%-81.5%，the activity of catalase wasincreased by8.4%-40.4%，and the activity of peroxidase was increased by 1.19%-18.5%.The high -throughput sequencing analysis results showed that the application of complex oligosaccharides increased the abundance of Sphingomonassp.and Methylobacteriumsp.in the leaf microbialcommunity，both of which have the effects of promoting plant growth and assisting plant diseaseresistance.Inaddition，complex oligosaccharides increased the abundance of biocontrol bacteria Bacillussp.in the microbial community of the rhizospheresoil.The application of complex oligosaccharides in the early and growth stages of cucumber could increase cucumber yield and plant antioxidant enzymeactivity，delay plantaging，and increase the abundance of beneficial bacteria in the plant growthsystem. It could provide basic data for further revealing the mechanism of action of complex oligosaccharides.